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ST1511 Anti-FGF1 Mouse mAb (3F5)

Anti-FGF1 Mouse mAb (3F5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Fibroblast Growth Factor 1, Acidic

ST1511
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the FGF1 protein in HeLa cells.
Catalogue NumberST1511
Brand Family Calbiochem®
SynonymsAnti-Fibroblast Growth Factor 1, Acidic
Application Data
Detection of human FGF1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-FGF1 Mouse mAb (3F5) (Cat. No. ST1511) (1-5 µg/ml). Detection: chemilimunescence.

Detection of human FGF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-FGF1 Mouse mAb (3F5) (Cat. No. ST1511) (10 µg/ml). Detection: fluorescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Imunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 46-155 of human FGF1, expressed as a GST fusion protein
Clone3F5
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1511 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Fibroblast Growth Factor 1, Acidic
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Imunocytochemistry (10 µg/ml)
Application Data
Detection of human FGF1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-FGF1 Mouse mAb (3F5) (Cat. No. ST1511) (1-5 µg/ml). Detection: chemilimunescence.

Detection of human FGF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-FGF1 Mouse mAb (3F5) (Cat. No. ST1511) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the FGF1 protein.
BackgroundFGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 46-155 of human FGF1, expressed as a GST fusion protein
Clone3F5
IsotypeIgG₁
Specieshuman
Positive controlHeLa cells
FormLiquid
FormulationIn PBS, pH 7.2.
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review