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GR39 Anti-Estrogen Receptor β (Ab-2) Mouse mAb (9.88)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ER β

GR39
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~53 kDa estrogen receptor β protein. Does not cross-react with estrogen receptor α.
Catalogue NumberGR39
Brand Family Calbiochem®
SynonymsAnti-ER β
Application Data
Detection of estrogen receptor β by immunoblotting. Samples: Recombinant human estrogen receptor β (100 ng) (Lane A), recombinant human estrogen receptor α (100 ng) (Lane B), total extract from mouse brain (Lane C), and whole cell lysate from PC12 cells (Lane D). Primary antibody: Anti-Estrogen Receptor β (Ab-2) Mouse mAb (9.88) (Cat. No. GR39) (1:1000). Detection: chemiluminescence.
References
ReferencesShughrue, P.J., et al. 1998. Endocrinol. 139, 5267.
Al-Shamma, H.A. and Arnold, A.P. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
Li, X., et al. 1997. Neuroendocrinol. 66, 63.
Pettersson, K., et al. 1997. Mol. Endocrinol. 11, 1486.
Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
Shughrue, P.J., et al. 1997. J. Comp. Neurol. 388, 507.
Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
Weigel, N.L. 1996. Biochem J. 319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
Product Information
FormLiquid
FormulationUndiluted ascites.
Positive controlRecombinant human estrogen receptor β protein, PC12 cells, or mouse brain
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Not Paraffin Sections
Application NotesImmunoblotting (1:1000)
Immunoprecipitation (1 µl)
Paraffin Sections (not recommended)
Application CommentsDoes not cross-react with estrogen receptor α protein. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human estrogen receptor β
ImmunogenHuman
Clone9.88
HostMouse
IsotypeIgM
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GR39 0

Documentation

Anti-Estrogen Receptor β (Ab-2) Mouse mAb (9.88) SDS

Title

Safety Data Sheet (SDS) 

Anti-Estrogen Receptor β (Ab-2) Mouse mAb (9.88) Certificates of Analysis

TitleLot Number
GR39

References

Reference overview
Shughrue, P.J., et al. 1998. Endocrinol. 139, 5267.
Al-Shamma, H.A. and Arnold, A.P. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
Li, X., et al. 1997. Neuroendocrinol. 66, 63.
Pettersson, K., et al. 1997. Mol. Endocrinol. 11, 1486.
Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
Shughrue, P.J., et al. 1997. J. Comp. Neurol. 388, 507.
Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
Weigel, N.L. 1996. Biochem J. 319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
SynonymsAnti-ER β
ApplicationImmunoblotting (1:1000)
Immunoprecipitation (1 µl)
Paraffin Sections (not recommended)
Application Data
Detection of estrogen receptor β by immunoblotting. Samples: Recombinant human estrogen receptor β (100 ng) (Lane A), recombinant human estrogen receptor α (100 ng) (Lane B), total extract from mouse brain (Lane C), and whole cell lysate from PC12 cells (Lane D). Primary antibody: Anti-Estrogen Receptor β (Ab-2) Mouse mAb (9.88) (Cat. No. GR39) (1:1000). Detection: chemiluminescence.
DescriptionMouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~53 kDa estrogen receptor β protein.
BackgroundSteroids regulate important neuronal functions including the organization of neural circuits during development and the regulation of synapse organization in the adult. Steroid hormones may promote cell survival and differentiation of both neurons and glial cells, regulate synaptogenesis, prevent synapse elimination, cause axonal and dendritic growth, and play a role during regeneration of the nervous system. Steroid hormone receptors are members of a large family of nuclear ligand-activated transcription factors that includes the androgen, estrogen, glucocorticoid, and progesterone receptors. In the absence of hormone, steroid receptors are associated in complexes with heat shock proteins and immunophilins. Steroid binding results in the dissociation of the heat-shock protein, receptor dimerization, and binding to specific hormone response elements (HRE) to produce a transcriptionally active complex that leads to the activation or repression of target genes. Steroid receptors are regulated by phosphorylation, however, modulation of kinase activity can also cause receptor activation in the absence of hormone. Recent evidence suggests that steroid receptor expression can be regulated by neurotrophins, and that steroids can regulate the expression of trophic factors and their receptors. In addition to the classical estrogen receptor α, a second estrogen receptor, designated estrogen receptor &beta, has been identified. Although initially identified from the rat prostate, estrogen receptor β is expressed in a variety of tissues including the brain. In vitro studies demonstrate that estrogen receptor α and estrogen receptor β form both homodimers and heterodimers. Additionally, in situ hybridization and immunohistochemical studies indicate that estrogen receptor α and β are coexpressed in certain neuronal populations, but also exhibit distinct non-overlapping expression in the nervous system.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human estrogen receptor β
Clone9.88
IsotypeIgM
Specieshuman, mouse, rat
Positive controlRecombinant human estrogen receptor β protein, PC12 cells, or mouse brain
FormLiquid
FormulationUndiluted ascites.
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with estrogen receptor α protein. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesShughrue, P.J., et al. 1998. Endocrinol. 139, 5267.
Al-Shamma, H.A. and Arnold, A.P. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
Li, X., et al. 1997. Neuroendocrinol. 66, 63.
Pettersson, K., et al. 1997. Mol. Endocrinol. 11, 1486.
Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
Shughrue, P.J., et al. 1997. J. Comp. Neurol. 388, 507.
Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
Weigel, N.L. 1996. Biochem J. 319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.