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PC283 Anti-ETO (Ab-1) Rabbit pAb

Anti-ETO (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MTG8

PC283
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes human ETO in HEL cells.
Catalogue NumberPC283
Brand Family Calbiochem®
SynonymsAnti-MTG8
References
ReferencesWang, J., et al. 1997. Cancer Res. 57, 2951.
Yergeau, D.A., et al. 1997. Nat. Genet. 15, 303.
Klampfer, L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14059.
Rhoades, K.L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11895.
Era, T., et al. 1995. Genes Chromosomes Cancer 13, 25.
Erickson, P.F., et al. 1994. Cancer Res. 54, 1782.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Positive controlHEL cells
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Not Frozen Sections
Not Immunofluorescence
Not Paraffin Sections
Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence)
Immunoprecipitation (1 µg/sample)
Frozen Sections (not recommended)
Paraffin Sections (not recommended)
Immunofluorescence (not recommended)
Application CommentsAntibody should be tritrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of the C-terminal 212 amino acids of human ETO
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC283 0

Documentation

Anti-ETO (Ab-1) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-ETO (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC283

References

Reference overview
Wang, J., et al. 1997. Cancer Res. 57, 2951.
Yergeau, D.A., et al. 1997. Nat. Genet. 15, 303.
Klampfer, L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14059.
Rhoades, K.L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11895.
Era, T., et al. 1995. Genes Chromosomes Cancer 13, 25.
Erickson, P.F., et al. 1994. Cancer Res. 54, 1782.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
SynonymsAnti-MTG8
ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence)
Immunoprecipitation (1 µg/sample)
Frozen Sections (not recommended)
Paraffin Sections (not recommended)
Immunofluorescence (not recommended)
DescriptionPurified rabbit polyclonal antibody. Recognizes the ETO protein.
BackgroundOne of the most frequent translocations seen in acute myeloid leukemias (AML) is that which occurs between the AML1 locus on chromosome 21 and a second locus on chromosome 8, the so called ETO/MTG8 (eight twenty one) locus. While the exact function of the normal ETO protein is unknown based on the high content of proline and threonine residues, the putative zinc fingers and the TAF110 homology domains, it is most likely that it functions as a transcription factor. The translocation between AML1 and ETO is observed in 40% of M2-subtype AML's . In the translocation the amino terminal end of AML1 becomes fused to an almost full length ETO protein. The resulting chimeric protein has multiple functions among which are the ability to transactivate the bcl-2 gene in U937 cells, and the M-CSF gene. Both of these activating functions are markedly different from the normal function of the fusion protein, which is the repression of transcription. The normal ETO protein may also has important functions on its own based on the observation of ETO transcripts in normal hematopoietic cells of different lineages and the presence of relatively high message levels in the developing brain as well as the observation that AML/ETO heterozygous mice die in midgestation from CNS hemorrhaging.
HostRabbit
Immunogen speciesHuman
Immunogena recombinant protein consisting of the C-terminal 212 amino acids of human ETO
IsotypeIgG
Specieshuman, mouse
Positive controlHEL cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be tritrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesWang, J., et al. 1997. Cancer Res. 57, 2951.
Yergeau, D.A., et al. 1997. Nat. Genet. 15, 303.
Klampfer, L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14059.
Rhoades, K.L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11895.
Era, T., et al. 1995. Genes Chromosomes Cancer 13, 25.
Erickson, P.F., et al. 1994. Cancer Res. 54, 1782.