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MAB2053 Anti-Disialoganglioside GD3 Antibody, clone MB3.6

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MAB2053
100 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IF, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2053
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Disialoganglioside GD3 Antibody, clone MB3.6
References
Product Information
FormatPurified
PresentationLiquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% NaN3.
Quality LevelMQ100
Applications
ApplicationAnti-Disialoganglioside GD3 Antibody, clone MB3.6 is an antibody against Disialoganglioside GD3 for use in FC, IF, IH.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry
Application NotesImmunohistochemistry (frozen tissue sections)

Immunofluorescence of cells in culture

Flow cytometry: 1-2 μg per 10E6 cells

Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).

Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.

TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}





Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenFM9 melanoma cells
CloneMB3.6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.
IsotypeIgG3
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB2053 04053252728174

Documentation

Anti-Disialoganglioside GD3 Antibody, clone MB3.6 SDS

Title

Safety Data Sheet (SDS) 

Anti-Disialoganglioside GD3 Antibody, clone MB3.6 Certificates of Analysis

TitleLot Number
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 3075012
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 - 2564847 2564847
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 MONOCLONAL ANTIBODY - 2127790 2127790
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 MONOCLONAL ANTIBODY - 2153567 2153567
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 - 3272290 3272290
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 - 3326737 3326737
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 - 3447258 3447258
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 - 3754816 3754816
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 - 3810483 3810483
MOUSE ANTI-HUMAN DISIALOGANGLIOSIDE GD3 - 3936239 3936239

References

Reference overviewPub Med ID
Characterization of new serum biomarkers in breast cancer using lipid microarrays.
Yoshiya Yonekubo,Ping Wu,Aimalohi Esechie,Yueqiang Zhang,Guangwei Du
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine  31  2010

Show Abstract
20393826 20393826
Analysis of effector cells in human antibody-dependent cellular cytotoxicity with murine monoclonal antibodies.
Ortaldo, JR et al.
J Immunol., 138(10):3566-3572 (1987)  1987

2437198 2437198
Biosynthesis and expression of the disialoganglioside GD2, a relevant target antigen on small cell lung carcinoma for monoclonal antibody-mediated cytolysis.
Cheresh, D A, et al.
Cancer Res., 46: 5112-8 (1986)  1986

Show Abstract
3019521 3019521