Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABC462 Anti-DNA polymerase subunit gamma-1 Antibody/POLG

This Anti-DNA polymerase subunit gamma-1 Antibody/POLG is validated for use in Immunohistochemistry and Immunoprecipitation for the detection of DNA polymerase subunit gamma-1/POLG.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: POLG, MDP1, POLG1, POLGA UniProt Number: P54098
View Products on Sigmaaldrich.com
ABC462
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC462
ReplacesMABN1088
DescriptionAnti-DNA polymerase subunit gamma-1 Antibody/POLG
Alternate Names
  • DNA polymerase subunit gamma-1
  • DNA polymerase subunit gamma-1
  • PolG-alpha
Background InformationPOLG is a mitochondrial protein that plays a major role in mitochondrial DNA replication and only for mitochondrial DNA. POLG is the catalytic subunit of a multiple protein complex that makes up the holo-enzyme that is the mitochondrial specific DNA polymerase gamma. POLG makes the alpha subunit of the complex. A number of diseases are associated with mutations that affect the function of POLG and the mitochondria including Leigh syndrome, various neurodegeneration diseases such as Parkinson’s and Alzheimer's, as well as various skeletal muscle and ophthalmoplegia disorders and even male infertility. The polymerase that POLG ultimately comprises is Polymerase gamma (Pol γ) and is the only DNA polymerase that is active in mitochondria and that can replicate mtDNA.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-DNA polymerase subunit gamma-1 Antibody/POLG is validated for use in Immunohistochemistry and Immunoprecipitation for the detection of DNA polymerase subunit gamma-1/POLG.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DNA polymerase subunit gamma-1/POLG in human cerebellum, human cerebral cortex, human tonsil and human thalamus tissue.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated DNA polymerase subunit gamma-1/POLG in 0.5 mg of HeLa cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human DNA polymerase subunit gamma-1/POLG near the N-terminus.
EpitopeNear N-terminus
Concentration1.0 mg/mL
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLG
  • MDP1
  • POLG1
  • POLGA
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~140 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human tonsil tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected DNA polymerase subunit gamma-1/POLG in human tonsil tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC462 04053252981821