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MABT322 Anti-DDR2 Antibody, clone 2B12.1

This Anti-DDR2 Antibody, clone 2B12.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of DDR2.

Anti-DDR2 Antibody, clone 2B12.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: DDR2, NTRKR3, TKT, TYRO10 UniProt Number: Q16832
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MABT322
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT322
DescriptionAnti-DDR2 Antibody, clone 2B12.1
Alternate Names
  • Discoidin domain-containing receptor 2
  • CD167 antigen-like family member B
  • CD167b
  • Discoidin domain receptor 2
  • Discoidin domain-containing receptor tyrosine kinase 2
  • Neurotrophic tyrosine kinase, receptor-related 3
  • Receptor protein-tyrosine kinase TKT
  • Tyrosine-protein kinase TYRO10
Background InformationDiscoidin domain-containing receptor 2 (EC 2.7.10.1; UniProt Q16832; also known as CD167 antigen-like family member B, CD167b, Discoidin domain receptor 2, Discoidin domain-containing receptor tyrosine kinase 2, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10) is encoded by the DDR2 (also known as NTRKR3, TKT, TYRO10) gene (Gene ID 4921) in human. Discoidin domain-containing receptors (DDRs) belong to the superfamily of transmembrane receptor tyrosine kinases (RTKs). DDRs are distinguished from other RTKs by their extracellular discoidin motif and their utilization of collagens as internal ligands. While DDR1 binds essentially all types of collagens, DDR2 shows a preference for type I, II and III fibrillar collagens, and nonfibrillar type X collagen. DDR1 is widely expressed in epithelial cells in lung, kidney, colon, and brain, whereas DDR2 is primarily expressed in mesenchymal cells including fibroblasts, myofibroblasts, smooth muscle cells, and chondrocytes in kidney, skin, lung, heart, and connective tissues. DDRs are important mediators of fundamental cellular processes, such as proliferation, survival, differentiation, adhesion and matrix remodeling, their dysregulations are reported to cause a number of human diseases, including fibrotic disorders, atherosclerosis and cancer. DDR2 is initially produced with a signal peptide sequence (a.a. 1-21), the removal of which yields the mature protein with an extracellular region (a.a. 22-399), followed by a transmembrane segment (a.a. 400-421) and a cytoplasmic region (a.a. 422-855) that harbors the kinase domain (a.a. 563-849).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-DDR2 Antibody, clone 2B12.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of DDR2.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 1.0 µg/mL of this antibody detected DDR2 in 10 µg of rat L6 myoblast and human HeLa cell lysates.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected DDR2 in human lung and kidney tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human DDR2 internal fragment.
EpitopeInternal (C-terminal half).
Clone2B12.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DDR2
  • NTRKR3
  • TKT
  • TYRO10
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~107/115 kDa observed. Target band(s) appears larger than the calculated molecular weight of 96.74 kDa due to glycosylation. A doublet banding pattern is often seen with the lower band representing less glycosylated DDR2 species.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DDR2 in 10 µg of NIH3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT322 04055977314960

Documentation

Anti-DDR2 Antibody, clone 2B12.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-DDR2 Antibody, clone 2B12.1 Certificates of Analysis

TitleLot Number
Anti-DDR2, clone 2B12.1 - 3393255 3393255
Anti-DDR2, clone 2B12.1 - 3789985 3789985
Anti-DDR2, clone 2B12.1 - 3880376 3880376
Anti-DDR2, clone 2B12.1 - 3943794 3943794
Anti-DDR2, clone 2B12.1 - 4057488 4057488
Anti-DDR2, clone 2B12.1 - 4107718 4107718
Anti-DDR2, clone 2B12.1 -Q2642748 Q2642748
Anti-DDR2, clone 2B12.1 Monoclonal Antibody 3156730