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MABT1490-50UL Anti-Cytokeratin-7 Antibody, clone 7D3

Anti-Cytokeratin-7, clone 7D3, Cat. No. MABT1490, is a rat monoclonal antibody that detects cytokeratin-7 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Frozen), and Immunohistochemistry (Paraffin).

MABT1490-50UL
50 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1490-50UL
DescriptionAnti-Cytokeratin-7 Antibody, clone 7D3
Alternate Names
  • Keratin
  • type II cytoskeletal 7
  • Cytokeratin-7
  • CK-7
  • Keratin-7
  • K7
  • Sarcolectin
  • Type-II keratin Kb7
Background InformationKeratin, type II cytoskeletal 7 (UniProt: P08729; also known as Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7) is encoded by the KRT7 (also known as SCL) gene (Gene ID: 3855) in human. Cytokeratins are intermediate filament proteins present in epithelial cells. They are expressed in a tissue-specific manner in normal organs and the tumors that arise from them. CK-7 is a type II basic low molecular weight cytokeratin that is found in simple epithelia in a variety of organs. Its expression is reported in bronchial and mesothelial cells, but is absent in colon, ectocervix, and liver. It is also observed throughout the glandular cells in the junction between stomach and esophagus. However, it is absent in the esophagus. CK-7 is also typically expressed in tumor cells of mammary and extramammary Paget disease. CK-7 expression can be used to distinguish ovarian and gastrointestinal carcinomas, or transitional cell carcinomas and prostate cancer.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Cytokeratin-7, clone 7D3, Cat. No. MABT1490, is a rat monoclonal antibody that detects cytokeratin-7 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Frozen), and Immunohistochemistry (Paraffin).
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:25 dilution from a representative lot detected Cytokeratin-7 in human breast cancer tissue sections.

Immunocytochemistry Analysis: A representative lot detected Cytokeratin-7 in Immunocytochemistry applications (Zydek, M., et. al. (2014). Viruses. 6(3):1346-64; Tabata, T., et. al. (2015). J Virol. 89(9):5134-47; Maidji, E., et. al. (2002). Virology. 304(1):53-69).

Immunohistochemistry Analysis: A representative lot detected Cytokeratin-7 in Immunohistochemistry applications (Zhou, Y., et. al. (2014). Am J Pathol. 184(12):3321-31; Schanz, A., et. al. (2014). Placenta. 35(12):1089-94; Genbacev, O., et. al. (2000). Am J Pathol. 157(4):1337-51).
Biological Information
ImmunogenWhole cytotrophoblast cells from 1st trimester human placenta.
Epitopeextracellular domain
Clone7D3
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 7D3 is a rat monoclonal antibody that detects human Cytokeratin-7. It targets an epitope with in the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KRT7
  • SCL
Purification MethodProtein G purified
UniProt Number
Molecular Weight51.38 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human pancreatic cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected Cytokeratin-7 in human pancreatic cancer tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1490-50UL 04054839646782