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AP1035 Anti-Cyclophilin D Mouse mAb (E11AE12BD4)

This Anti-Cyclophilin D Mouse mAb (E11AE12BD4) is validated for use in Immunoblotting, Immunocytochemistry for the detection of Cyclophilin D.

Anti-Cyclophilin D Mouse mAb (E11AE12BD4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CyPD

AP1035
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~17-18 kDa processed form of mitochondrial cyclophilin D in cultured fibroblasts.
Catalogue NumberAP1035
Brand Family Calbiochem®
SynonymsAnti-CyPD
Application Data
Detection of human cyclophilin D by immunoblotting. Sample: Heart mitochondria. Primary antibody: Anti-Cyclophilin D Mouse mAb (E11AE12BD4) (Cat. No. AP1035) (1 μg/ml). Detection: chemiluminescence.

Detection of human cyclophilin D by immunocytochemistry. Sample: Cultured fibroblasts. Primary antibody: Anti-Cyclophilin D Mouse mAb (E11AE12BD4) (Cat. No. AP1035) (5 μg/ml). Detection: fluorescence.
References
ReferencesHalestrap, A.P., et al. 2003. Curr. Med. Chem. 10, 1507.
Product Information
FormLiquid
FormulationIn HBS.
Positive controlHeart mitochondria
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1 µg/ml)
Immunocytochemistry (5 µg/ml)
Application CommentsUseful for detection of apoptosis related proteins. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, rat cyclophilin D
ImmunogenRat
CloneE11AE12BD4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1035 0

Documentation

Anti-Cyclophilin D Mouse mAb (E11AE12BD4) SDS

Title

Safety Data Sheet (SDS) 

Anti-Cyclophilin D Mouse mAb (E11AE12BD4) Certificates of Analysis

TitleLot Number
AP1035

References

Reference overview
Halestrap, A.P., et al. 2003. Curr. Med. Chem. 10, 1507.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-February-2010 JSW
SynonymsAnti-CyPD
ApplicationImmunoblotting (1 µg/ml)
Immunocytochemistry (5 µg/ml)
Application Data
Detection of human cyclophilin D by immunoblotting. Sample: Heart mitochondria. Primary antibody: Anti-Cyclophilin D Mouse mAb (E11AE12BD4) (Cat. No. AP1035) (1 μg/ml). Detection: chemiluminescence.

Detection of human cyclophilin D by immunocytochemistry. Sample: Cultured fibroblasts. Primary antibody: Anti-Cyclophilin D Mouse mAb (E11AE12BD4) (Cat. No. AP1035) (5 μg/ml). Detection: fluorescence.
DescriptionMouse monoclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~17-18 kDa processed form of mitochondrial cyclophilin D (CyPD) protein.
BackgroundCyclophilin D is a member of the immunophilin family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents, cyclosporin A (CsA) or FK506. Proteins that bind CsA are called cyclophilins. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPlase) whose activity is inhibited by the respective immunosuppressant compounds. Immunophilins accelerate folding of proteins in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins. Cyclophilin D is located in the intermembrane space of the mitochondria where it is an integral member of the permeability transition pore complex that includes the voltage dependent anion channel (VDAC) and adenine nucleotide translocase (ANT).
HostMouse
Immunogen speciesRat
Immunogenrecombinant, rat cyclophilin D
CloneE11AE12BD4
IsotypeIgG₁
Speciesbovine, human, mouse, rat
Positive controlHeart mitochondria
FormLiquid
FormulationIn HBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsUseful for detection of apoptosis related proteins. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHalestrap, A.P., et al. 2003. Curr. Med. Chem. 10, 1507.