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69050 Anti-Cre Antibody

Specific, sensitive detection of Cre recombinase in cells and cell lysates

Anti-Cre Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information
Description
OverviewRecombinase cre (UniProt: P06956; also known as Cre) is encoded by the cre gene (Gene ID: 2777477) in Escherichia phage P1 (Bacteriophage P1). Cre (Causes recombination with Cyclization recombinase), a 38 kDa DNA recombinase of the 'phage' integrase family, is derived from the P1 bacteriophage. It catalyzes site-specific recombination and is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each bind to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals in vivo.
Supplied as purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide and 50% glycerol.

Evaluated by Western Blotting with Recombinant Cre-His.

Western Blotting Analysis: Representative lot data. Recombinant Cre-His loaded at 20 ng/lane (Lane 1) and 10 ng/lane (Lane 2) was probed with Cat. No. 69050-3, Anti-Cre (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Cre (~39 kDa).
Catalogue Number69050
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69050 0

Documentation

Anti-Cre Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Cre Antibody Certificates of Analysis

TitleLot Number
69050

Citations

Title
  • Qian, J.Y., et al. 2008. Hypertension 51, 560.
  • Dworniczak, B., et al. 2007. Nephron Experimental Nephrology 106, e11.
  • Froment, P., et al. 2007. Journal of Endocrinology 194, 557.
  • Tiedt, R., et al. 2007. Blood 109, 1503.
  • Davé, V., et al. 2006. Journal of Clinical Investigation 116, 2597.
  • Forni, P.E., et al. 2006. Journal of Neuroscience 26, 9593.
  • Gao, F., et al. 2006. Procedings of the National Academy of Science 103, 11987.
  • Masahira, N., et al. 2006. Developmental Biology 293, 358.
  • Rajji, T., et al. 2006. Journal of Neuroscience 26, 908.
  • Wei, Q., et al. 2006. Cancer Research 66, 7429.
  • Xie, y., et al. 2006. Journal of Biological Chemistry 281, 4075.
  • Balthasar N., et al. 2005. Cell 123, 493.
  • Izumi, T., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 5739.
  • Koike, C., et al. 2005. Journal of Neuroscience 25, 10290.
  • Moeller, M.J., et al. 2005. American Journal of Physiology: Renal 289, F481.
  • Pfister, O., et al. 2005. Circulation Research 97, 52.
  • Schulein, R., et al. 2005. Procedings of the National Academy of Science 102, 856.
  • Peitz, M., et al. 2002. Procedings of the National Academy of Science 99, 4489.
  • Takebayashi, H., et al. 2002. Current Biology 12, 1157.
  • Morimura, T., et al. 2000. Journal of Biological Chemistry 275, 36523.
  • Selbert, S., et al. 1998. Transgenic Research 7, 387.
  • Giovannini, et. al. 2000. Genes and Development 14, 1617.
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    User Protocols

    Title
    TB421 Anti-Cre Antibody