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PC645 Anti-Cleaved Bid (Ab-1) Rabbit pAb

Anti-Cleaved Bid (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC645
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
MRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~15 kDa cleaved Bid protein. Does not recognize uncleaved, full-length Bid.
Catalogue NumberPC645
Brand Family Calbiochem®
Application Data
Detection of mouse cleaved Bid by immunoblotting. Samples: Whole cell lysate from L929 cells left untreated (lane 1), treated with actinomycin alone (5 µg/ml, lane 2), or treated with 5 µg/ml actinomycin plus 2.5 ng/ml, 5 ng/ml, and 10 ng/ml mouse TNFα (lanes 3, 4, 5, respectively). Primary antibody: Anti-Cleaved Bid (Ab-1) Rabbit pAb (Cat. No. PC645) (right panel) (0.5 µg/ml) and Anti-Bid total left panel (5 µg/ml). Detection: chemiluminescence.
References
ReferencesEskes, R., et al. 2000. Mol. Cell. Biol. 20, 929.
Chou, J.J., et al. 1999. Cell 96, 615.
Desagher, S., et al. 1999. J. Cell Biol. 144, 891.
Gross, A., et al. 1999. J. Biol. Chem. 274, 1156.
Kluck, R.M., et al. 1999. J. Cell Biol. 147, 809.
Luo, X., et al. 1999. Cell 94, 481.
Tan, K.O., et al. 1999. J. Biol. Chem 274, 23687.
Footz, T.K., et al. 1998. Genomics 51, 472.
Wang, K., et al. 1996. Genes Dev. 10, 2859.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg+2 and Ca+2), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Positive controlTNF-α-treated L929 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000, chemiluminescence)
Application CommentsAt a 1:1000 dilution there is sufficient antibody to perform 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids at the N-terminus of the 59/60 cleavage site of mouse Bid (BH3 interacting domain death agonist)
ImmunogenMouse
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC645 0

Documentation

Anti-Cleaved Bid (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC645

References

Reference overview
Eskes, R., et al. 2000. Mol. Cell. Biol. 20, 929.
Chou, J.J., et al. 1999. Cell 96, 615.
Desagher, S., et al. 1999. J. Cell Biol. 144, 891.
Gross, A., et al. 1999. J. Biol. Chem. 274, 1156.
Kluck, R.M., et al. 1999. J. Cell Biol. 147, 809.
Luo, X., et al. 1999. Cell 94, 481.
Tan, K.O., et al. 1999. J. Biol. Chem 274, 23687.
Footz, T.K., et al. 1998. Genomics 51, 472.
Wang, K., et al. 1996. Genes Dev. 10, 2859.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-April-2009 RFH
ApplicationImmunoblotting (1:1000, chemiluminescence)
Application Data
Detection of mouse cleaved Bid by immunoblotting. Samples: Whole cell lysate from L929 cells left untreated (lane 1), treated with actinomycin alone (5 µg/ml, lane 2), or treated with 5 µg/ml actinomycin plus 2.5 ng/ml, 5 ng/ml, and 10 ng/ml mouse TNFα (lanes 3, 4, 5, respectively). Primary antibody: Anti-Cleaved Bid (Ab-1) Rabbit pAb (Cat. No. PC645) (right panel) (0.5 µg/ml) and Anti-Bid total left panel (5 µg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~15 kDa cleaved mouse Bid.
BackgroundBid (~22 kDa) is a BH3 domain containing pro-apoptotic cytosolic protein that maps to human chromosome 22q11.2. Initially localized to the cytoplasm, the Bid protein is cleaved by the action of caspase 8 into fragments of two different sizes, p15 and p13 and becomes inserted into the mitochondrial membrane. Of the two fragments the p15 BH3 containing fragment is the more potent inducer of apoptosis and in fact is a stronger inducer than the full-length Bid protein. Following cleavage by caspase 8, Bid will bind with Bax localizing it to the mitochondrial membrane and leading to the release of cyto-chrome c. This interaction is blocked by Bcl-x, which directly binds to Bax, competing for the binding of Bax to the membrane-localized Bid.
HostRabbit
Immunogen speciesMouse
Immunogena synthetic peptide corresponding to amino acids at the N-terminus of the 59/60 cleavage site of mouse Bid (BH3 interacting domain death agonist)
IsotypeIgG
Speciesnot human, mouse
Positive controlTNF-α-treated L929 cells
FormLiquid
FormulationIn Dulbecco's PBS (without Mg+2 and Ca+2), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Preservative≤0.1% sodium azide
CommentsAt a 1:1000 dilution there is sufficient antibody to perform 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesEskes, R., et al. 2000. Mol. Cell. Biol. 20, 929.
Chou, J.J., et al. 1999. Cell 96, 615.
Desagher, S., et al. 1999. J. Cell Biol. 144, 891.
Gross, A., et al. 1999. J. Biol. Chem. 274, 1156.
Kluck, R.M., et al. 1999. J. Cell Biol. 147, 809.
Luo, X., et al. 1999. Cell 94, 481.
Tan, K.O., et al. 1999. J. Biol. Chem 274, 23687.
Footz, T.K., et al. 1998. Genomics 51, 472.
Wang, K., et al. 1996. Genes Dev. 10, 2859.