Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB13170 Anti-Cav1.2 calcium channel Antibody, clone L57/46

This Anti-Cav1.2 calcium channel Antibody, clone L57/46 is validated for use in WB, IH for the detection of Cav1.2 calcium channel.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: CACH2, CACN2, TS, CACNL1A1, CaV1.2, CCHL1A1, Cav1.2 UniProt Number: P15381
View Products on Sigmaaldrich.com
MAB13170
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, R, RbWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB13170
DescriptionAnti-Cav1.2 calcium channel Antibody, clone L57/46
Alternate Names
  • calcium channel, voltage-dependent, L type, alpha 1C subunit1
  • voltage-gated calcium channel alpha subunit Cav1.2
  • calcium channel, L type, alpha 1 polypeptide, isoform 1, cardic muscle
  • calcium channel, cardic dihydropyridine-sensitive, alpha-1 subunit
  • voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit, splice variant 10*
  • DHPR, alpha-1 subunit
  • Voltage-gated calcium channel subunit alpha Cav1.2
  • Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle
Background InformationThis gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
References
Product Information
FormatPurified
Control
  • Mouse brain tissue lysate
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Cav1.2 calcium channel Antibody, clone L57/46 is validated for use in WB, IH for the detection of Cav1.2 calcium channel.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Cav1.2 calium channel in human kidney tissue.
Biological Information
ImmunogenRecombinant protein corresponding to rabbit Cav1.2 calcium channel at the cytoplasmic domain proximal to the C-terminus.
EpitopeCytoplasmic domain
CloneL57/46
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes Cav1.2 calcium channel at the cytoplasmic domain.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
  • Human
  • Rat
  • Rabbit
Species Reactivity NoteDemonstrated to react with mouse and human. Predicted to react with rat and rabbit based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CACH2
  • CACN2
  • TS
  • CACNL1A1
  • CaV1.2
  • CCHL1A1
  • Cav1.2
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function (By similarity).

SUBUNIT STRUCTURE: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with CABP1 and CACNA2D4 By similarity. Interacts (via C-terminal CDB motif) with CABP5; in a calcium-dependent manner (By similarity).

SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Cell membrane. Note: The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane. TISSUE SPECIFICTY: Expression in cardiac muscle. In lung, expressed in airway and vascular smooth muscle cells.

DOMAIN: Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.

PTM: Phosphorylation by PKA activates the channel.

SEQUENCE SIMILARITIES: Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. (View classification).
Molecular Weight Observed at ~ 240 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Cav1.2 calcium channel on 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB13170 04053252518690

Documentation

Anti-Cav1.2 calcium channel Antibody, clone L57/46 SDS

Title

Safety Data Sheet (SDS) 

Anti-Cav1.2 calcium channel Antibody, clone L57/46 Certificates of Analysis

TitleLot Number
Anti-Cav1.2 calcium channel, clone L57/46 - 2127821 2127821
Anti-Cav1.2 calcium channel, clone L57/46 - 2394046 2394046
Anti-Cav1.2 calcium channel, -2550884 2550884
Anti-Cav1.2 calcium channel, -2579911 2579911
Anti-Cav1.2 calcium channel, -2667314 2667314
Anti-Cav1.2 calcium channel, -2716676 2716676
Anti-Cav1.2 calcium channel, -2727221 2727221
Anti-Cav1.2 calcium channel, -2762311 2762311
Anti-Cav1.2 calcium channel, -2807063 2807063
Anti-Cav1.2 calcium channel, clone L57/46 3030288
Show more

Brochure

Title
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.