Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN2330-25UG Anti-Catenin delta-2 Antibody, clone 4H2.1

Anti-Catenin delta-2, clone 4H2.1 Antibody, Cat. No. MABN2330, is a highly specific mouse monoclonal antibody that targets Catenin delta-2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Catenin delta-2 Antibody, clone 4H2.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: CTNND2, NPRAP UniProt Number: Q9UQB3
View Products on Sigmaaldrich.com
MABN2330-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2330-25UG
DescriptionAnti-Catenin delta-2 Antibody, clone 4H2.1
Alternate Names
  • Delta-catenin
  • GT24
  • Neural plakophilin-related ARM-repeat protein
  • NPRAP
  • Neurojungin
Background InformationCatenin delta-2 (UniProt: Q9UQB3; also known as Delta-catenin, GT24, Neural plakophilin-related ARM-repeat protein, NPRAP, Neurojungin) is encoded by the CTNND2 (also known as NPRAP) gene (Gene ID: 1501) in human. Delta Catenin is a component of the cadherin-catenin cell adhesion complex and is enriched in synaptosomes. Catenin Delta 2 is a member of the beta-catenin superfamily and is expressed in the brain where it is required for normal cognitive development and its highest expression is observed in fetal brain. Catenin Delta 2 is phosphorylated by Cdk5 and by GSK-3beta. Catenin Delta 2 plays a critical role in neuronal development, particularly in the formation and/or maintenance of dendritic spines and synapses. It is also involved in the regulation of Wnt signaling where it acts on beta-catenin turnover, facilitating beta-catenin interaction with GSK3beta, phosphorylation, ubiquitination, and degradation. When bound to ZBTB33 (Zinc Finger and BTB Domain Containing 33), it functions as a transcriptional activator. Catenin delta-2 also promotes the disruption of E-cadherin based adherens junction to favor cell spreading upon stimulation by hepatocyte growth factor. Two isoforms of Catenin delta-2 have been reported that are produced by alternative splicing. It has nine Armadillo (ARM) repeats and a coiled coil region (aa 49-84). Defects in CTNND2 gene are known to cause autism and delayed acquisition of psychomotor skills. (Ref.: Yuan, L et al. (2015). J. Biol. Chem. 290(17):10947-57).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Catenin delta-2, clone 4H2.1 Antibody, Cat. No. MABN2330, is a highly specific mouse monoclonal antibody that targets Catenin delta-2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Catenin delta-2 in rat thalamus, rat cerebellum, and human cerebral cortex tissue sections.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 145 amino acids from the C-terminal region of human Catenin delta-2.
Clone4H2.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4H2.1 specifically detects Catenin delta-2 in human and murine cells. It targets an epitope with in the C-tereminal region.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CTNND2
  • NPRAP
Purification MethodProtein G purified
UniProt Number
Molecular Weight~150 kDa observed; 132.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected Catenin delta-2 in mouse cerebellum tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2330-25UG 04054839507175