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ABC962 Anti-CDC45

Detect CDC45 using this rabbit polyclonal Anti-CDC45 Antibody, Cat. No. ABC962, validated for use in Immunocytochemistry and Western Blotting.

Anti-CDC45 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: CDC45, CDC45L, CDC45L2, UNQ374/PRO710 UniProt Number: O75419
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ABC962
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC962
DescriptionAnti-CDC45
Alternate Names
  • Cell division control protein 45 homolog
  • PORC-PI-1
Background InformationCell division control protein 45 homolog (UniProt O75419; also known as PORC-PI-1) is encoded by the CDC45 (also known as CDC45L, CDC45L2, UNQ374/PRO710) gene (Gene ID 8318) in human. Recruitment of replication initiation proteins at the G1/S transition enables origin DNA unwinding and loading of DNA polymerases. CDC45 is a replication initiation protein that plays an essential role in the establishment and progression of the replication fork. Cdc45 associates with the heterohexameric Mcm2-7 helicase and the GINS complex (go-ichi-nii-san, Japanese for 5-1-2-3, for the subunits Sld5, Psf1, Psf2 and Pfs3) to form the CMG (Cdc45-Mcm2-7-GINS) complex. CMG represents the eukaryotic replicative DNA helicase that unwinds origins of replication and drives the replication fork. CDC45 is also reported to interact with several other replication proteins, including DNA helicase B (DHB), Mcm10, Orc2, TopPB1, and DNA polymerases alpha, delta and epsilon.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect CDC45 using this rabbit polyclonal Anti-CDC45 Antibody, Cat. No. ABC962, validated for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CDC45 in L929 murine fibroblasts.
Biological Information
ImmunogenGST-tagged recombinant human CDC45 C-terminal fragment.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects CDC45 C-terminal region highly conserved among human, mouse, rat, and hamster species.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Hamster and Rat based on high sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDC45
  • CDC45L
  • CDC45L2
  • UNQ374/PRO710
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~75 kDa observed. 65.57/60.25/68.77 kDa (human isoform 1/2/3), 65.38 kDa (mouse) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in L929 fibroblast lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected CDC45 in 10 µg of L929 murine fibroblast lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC962 04054839052941