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MABF420 Anti-CD4 Antibody (mouse), APC-Cy7, clone RM4-5

This rat monoclonal antibody Anti-CD4 (mouse), APC-Cy7, clone RM4-5 validated for use in IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Cd4 UniProt Number: P06332
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MABF420
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIP, IHC, IF, FCRAPC-Cy7Monoclonal Antibody
Description
Catalogue NumberMABF420
DescriptionAnti-CD4 Antibody (mouse), APC-Cy7, clone RM4-5
Alternate Names
  • T-cell surface glycoprotein CD4
  • T-cell differentiation antigen L3T4
  • T-cell surface antigen T4/Leu-3
  • CD4
Background InformationThe RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will “compete” for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
References
Product Information
FormatAPC-Cy7
PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal antibody Anti-CD4 (mouse), APC-Cy7, clone RM4-5 validated for use in IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD4.
Key Applications
  • Immunoprecipitation
  • Immunohistochemistry
  • Immunofluorescence
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneRM4-5
Concentration0.2 mg/mL
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Cd4
Purification MethodSize Exclusion Chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg of this antibody detected CD4 in one million C57Bl/6 splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF420 04053252960116

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies