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MABF3243-100UG Anti-CD1d Antibody, clone CD1d42

Anti-CD1d, clone CD1d42 , Cat. No. MABF3243, is a mouse monoclonal antibody that detects CD1d and is tested for use in Flow Cytometry and Inhibition Assay.

MABF3243-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3243-100UG
DescriptionAnti-CD1d Antibody, clone CD1d42
Alternate Names
  • Antigen-presenting glycoprotein CD1d
  • R3G1
Background InformationAntigen-presenting glycoprotein CD1d (UniProt: P15813; also known as R3G1, CD1d) is encoded by the CD1D gene (Gene ID: 912) in human. Cd1d is a single-pass type 1 membrane glycoprotein, which is synthesized with a signal peptide (aa 1-19) that is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 20-301), a transmembrane domain (aa 302-322), and a short cytoplasmic domain (aa 323-335). It is expressed at high levels by human cortical thymocytes, and its expression is also observed on certain T cell leukemias. It serves as a key antigen-presenting molecule involved in the selection and activation of NK cells. It is a major-histocompatibility-complex (MHC) class-I-like molecule that presents self-lipid and non-self-lipid antigens to invariant natural killer T (iNKT) cells. Following engagement of the invariant T cell receptor (iTCR) by CD1d lipid complexes, iNKT cells proliferate, produce cytokines, and become cytotoxic, regulating innate and adaptive immune responses. (Ref.: Oleinika, K., et al. (2018). Nat. Commun. 9(1); Article 684; Spada, FM., et al. (2000). Eur. J. Immunol. 30; 3468-3477; Exley, M., et al. (2000). Immunology. 100(1): 37-47).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-CD1d, clone CD1d42 , Cat. No. MABF3243, is a mouse monoclonal antibody that detects CD1d and is tested for use in Flow Cytometry and Inhibition Assay.
Key Applications
  • Flow Cytometry
  • Inhibition
Application NotesTested applications

Inhibition Assay: A representative lot inhibited the responses of NK T cell clones to -GalCer in the presence of monocyte lineage APC. (Spada, F.M., et al. (2000). Eur J Immunol. 30(12):3468-77).

Flow Cytometry Analysis: A representative lot detected CD1d in Flow Cytometry (Spada, F.M., et al. (2000). Eur J Immunol. 30(12):3468-77).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant fusion protein composed of 1, 2 and 3 domains of human CD1d fused to the Fc portion of murine IgG2a.
EpitopeExtracellular domain
CloneCD1d42
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CD1d42 is a mouse monoclonal antibody that detects CD1d. It targets an epitope within the extracellular domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD1D
Purification MethodProtein G purified
UniProt Number
Molecular Weight37.72 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD1d in one million Human peripheral blood mononuclear cells (PBMC).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3243-100UG 04065269126072