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ABT69 Anti-CCDC53 Antibody

This Anti-CCDC53 Antibody is validated for use in WB for the detection of CCDC53.

Anti-CCDC53 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: CCDC53, CGI-116 UniProt Number: Q9Y3C0
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ABT69
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABT69
DescriptionAnti-CCDC53 Antibody
Alternate Names
  • coiled-coil domain containing 53
  • Coiled-coil domain-containing protein 53
  • WASH complex subunit CCDC53
Background InformationCoiled-coil domain-containing protein 53 (CCDC53) is one of several components comprising the WASH complex, a complex localized to endosomal surfaces that functions to upregulate actin polymerization through the recruiting and activation of Arp2/3 complex. WASH is a critical component in the fission of tubules (transport intermediates involved in the distribution of endosomes). The WASH complex is composed of CCDC53, F-actin-capping protein subunits alpha and beta, WASH, FAM21, KIAA1033, and KIAA196.
References
Product Information
FormatPurified
Control
  • HepG2 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-CCDC53 Antibody is validated for use in WB for the detection of CCDC53.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: 2.5 µg/mL from a representative lot detected CCDC53 in 10 µg of mouse brain and PC12 cell lysate.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human CCDC53.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CCDC53
  • CGI-116
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting.

SUBUNIT STRUCTURE: Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53.

SEQUENCE SIMILARITIES: Belongs to the CCDC53 family.
Molecular Weight~28 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 2.5 µg/mL of this antibody detected CCDC53 in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT69 04053252642630