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AB1534 Anti-Brain Derived Neurotrophic Factor Antibody

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AB1534
50 µL  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, HELISA, IHC, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberAB1534
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Brain Derived Neurotrophic Factor Antibody
Alternate Names
  • brain-derived neurotrophic factor
Background InformationBrain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer’s and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.
Product Information
FormatSerum
Control
  • Brain tissue
PresentationRabbit polyclonal antiserum in buffer containing no preservatives. Lyophilized. Reconstitute with 50 μL of sterile distilled water.
Quality LevelMQ100
Applications
ApplicationThis Anti-Brain Derived Neurotrophic Factor Antibody is validated for use in ELISA, IH, WB for the detection of Brain Derived Neurotrophic Factor.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Western Blotting
Applications Not Recommended
  • Inhibits Activity/Function
Application NotesImmunohistochemistry:
1:1000-1:2000 dilution of a previous lot was used in immunohistochemistry (see suggested protocol).

ELISA:
1:1000-1:2000 dilution of a previous lot was used in ELISA.

Bioassay:
Does not inhibit BDNF biological activity.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenA 10 amino acid peptide corresponding to the amino-terminal of mouse Brain Derived Neurotrophic Factor (BDNF) coupled to ovalbumin.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityBrain Derived Neurotrophic Factor (BDNF). By dot blot the antibody shows less than 0.1% cross reactivity with recombinant human NT3, NT4/5 or mouse NGF.
Species Reactivity
  • Mouse
  • Rat
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
Gene Symbol
  • BDNF
  • Abrineurin
  • neurotrophin
  • MGC34632
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
SIZE: 247 amino acids; 27818 Da
SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
SUBCELLULAR LOCATION: Secreted.
PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
Molecular Weight18 kDa, with a homodimer at ~27-30 kDa.
Product Usage Statements
Quality AssuranceEvaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected BDNF on 10 µg of mouse brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Global Trade Item Number
Catalogue Number GTIN
AB1534 04053252738487