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PC68 Anti-Bcl-2 (Ab-2) (20-34) Rabbit pAb

Anti-Bcl-2 (Ab-2) (20-34) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC68
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, OpRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~24-26 kDa Bcl-2 protein in MCF-7 cells, intestine, and lymph node tissue.
Catalogue NumberPC68
Brand Family Calbiochem®
References
ReferencesKozopas, K.M. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
Silverman, G.A., et al. 1993. Mol. Cell. Biol. 13, 5469.
Vaux, D.L. 1993. Proc. Natl. Acad. Sci.USA 90, 786.
Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
Product Information
FormLiquid
FormulationIn PBS.
Negative controlBrain tissue
Positive controlHuman intestine or lymph node tissue or purified Bcl-2 (Cat. No. PF126)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen sections (5 µg/ml)
Immunoblotting (5 µg/ml)
Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
Application CommentsBrain tissue may be used as a negative control, as only random cells stain positively. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (HYKLSQRGYEWDAGD) corresponding to amino acids 20-34 of human Bcl-2
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Opossum
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC68 0

Documentation

Anti-Bcl-2 (Ab-2) (20-34) Rabbit pAb Certificates of Analysis

TitleLot Number
PC68

References

Reference overview
Kozopas, K.M. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
Silverman, G.A., et al. 1993. Mol. Cell. Biol. 13, 5469.
Vaux, D.L. 1993. Proc. Natl. Acad. Sci.USA 90, 786.
Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Denis Grandgirard, et al. (1998) Alphaviruses induce apoptosis in Bcl-2-overexpressing cells: evidence for a caspase-mediated, proteolytic inactivation of Bcl-2. The EMBO Journal 17, (1268- 1278).
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision26-February-2008 JSW
    ApplicationFrozen sections (5 µg/ml)
    Immunoblotting (5 µg/ml)
    Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
    DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~24-26 kDa Bcl-2 protein.
    BackgroundThe bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a 24-26 kDa protein which has been localized to the inner mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death." It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock, and irradiation.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide (HYKLSQRGYEWDAGD) corresponding to amino acids 20-34 of human Bcl-2
    IsotypeIgG
    Specieshuman, mouse, opossum
    Positive controlHuman intestine or lymph node tissue or purified Bcl-2 (Cat. No. PF126)
    Negative controlBrain tissue
    FormLiquid
    FormulationIn PBS.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsBrain tissue may be used as a negative control, as only random cells stain positively. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesKozopas, K.M. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
    Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
    Silverman, G.A., et al. 1993. Mol. Cell. Biol. 13, 5469.
    Vaux, D.L. 1993. Proc. Natl. Acad. Sci.USA 90, 786.
    Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
    Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
    Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
    Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
    Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
    Citation
  • Denis Grandgirard, et al. (1998) Alphaviruses induce apoptosis in Bcl-2-overexpressing cells: evidence for a caspase-mediated, proteolytic inactivation of Bcl-2. The EMBO Journal 17, (1268- 1278).
    		•