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OP107 Anti-BRCA1 (Ab-4) Mouse mAb (SD118)

Anti-BRCA1 (Ab-4), mouse monoclonal, clone SD118, recognizes the ~220 kDa BRCA1 in HBL-100 and SK-BR3 cells. It is validated for WB, IF, IP, and for frozen sections.

Anti-BRCA1 (Ab-4) Mouse mAb (SD118) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Breast Cancer Susceptibility Protein

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OP107
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP107-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~220 kDa BRCA1 protein in HBL-100 and SK-BR3 cells. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
Catalogue NumberOP107
Brand Family Calbiochem®
SynonymsAnti-Breast Cancer Susceptibility Protein
References
ReferencesChen, J.J., et al. 1999. Cancer Res. 59, 1752s.
Cressman, V.L., et al. 1999. Cell Growth Differ. 10, 1.
Irminger-Finger, I., et al. 1999. Biol. Chem. 380, 117.
Xu, X., et al. 1999. Nat. Genet. 22, 37.
Wilson, C.A., et al. 1999. Nat. Genet. 21, 236.
Andres, J.L., et al. 1998. Oncogene 16, 2229.
Chen, J., et al. 1998. Mol. Cell 2, 317.
Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 5287.
Fan, S., et al. 1998. Int. J. Cancer 77, 600.
Gowen, L.C., et al. 1998. Science 281, 1009.
Hsu, L.C. and R.L. White. 1998. Proc. Natl. Acad. Sci. USA 95, 12983.
Shen, S.X., et al. 1998. Oncogene 17, 3115.
Tulchin, N., et al. 1998. Int. J. Oncol. 13, 513.
Ludwig, T., et al. 1997. Genes Dev 11, 1226.
Ruffner, H. and I.M. Verma. 1997. Proc. Natl. Acad. Sci. USA 94, 7138.
Scully, R., et al. 1997. Cell 90, 425.
Thakur, S., et al. 1997. Mol. Cell Biol. 17, 444.
Wilson, C.A., et al. 1997. Oncogene 14, 1.
Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin Oncol. 23, 1.
Chen, Y., et al. 1996. Cancer Res. 56, 3168.
Liu, C.Y., et al. 1996. Genes Dev. 10, 1835.
Gudas, J.M., et al. 1996. Cell Growth Differ. 7, 717.
Scully, R., et al. 1996. Science 272, 123.
Vaughn, J.P., et al. 1996. Cell Growth Differ. 7, 711.
Wilson, C.A. et al. 1996. Nat. Genet. 13, 264.
Thompson, M.E., et al. 1995. Nat. Genet. 9, 444.
Miki, Y., et al. 1994. Science 266, 66.
Product Information
DeclarationSold under license of U.S. Patents 5,753,441 and 6,162,897.
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlHBL-100 or SK-BR3 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting, Immunoprecipitation Wilson, C.A., et al. 1999. Nat. Genet. 21, 236. Original Clone Scully, R., et al. 1996. Science 272, 123.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Not Paraffin Sections
Application NotesFrozen Sections (2 µg/ml)
Immunoblotting (2 µg/ml, see application references)
Immunofluorescence (2 µg/ml)
Immunoprecipitation (2 µg, see application references)
Paraffin Sections (not recommended)
Application CommentsMaximal BRCA1 levels are observed in subconfluent cell populations after serum stimulation. Highly specific BRCA1 antibody useful for blotting and IP of full length BRCA1 protein. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant polypeptide corresponding to amino acids 1005-1313 in exon 11 of human BRCA1
ImmunogenHuman
CloneSD118
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP107-100UG 04055977224924

Documentation

Anti-BRCA1 (Ab-4) Mouse mAb (SD118) SDS

Title

Safety Data Sheet (SDS) 

Anti-BRCA1 (Ab-4) Mouse mAb (SD118) Certificates of Analysis

TitleLot Number
OP107

References

Reference overview
Chen, J.J., et al. 1999. Cancer Res. 59, 1752s.
Cressman, V.L., et al. 1999. Cell Growth Differ. 10, 1.
Irminger-Finger, I., et al. 1999. Biol. Chem. 380, 117.
Xu, X., et al. 1999. Nat. Genet. 22, 37.
Wilson, C.A., et al. 1999. Nat. Genet. 21, 236.
Andres, J.L., et al. 1998. Oncogene 16, 2229.
Chen, J., et al. 1998. Mol. Cell 2, 317.
Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 5287.
Fan, S., et al. 1998. Int. J. Cancer 77, 600.
Gowen, L.C., et al. 1998. Science 281, 1009.
Hsu, L.C. and R.L. White. 1998. Proc. Natl. Acad. Sci. USA 95, 12983.
Shen, S.X., et al. 1998. Oncogene 17, 3115.
Tulchin, N., et al. 1998. Int. J. Oncol. 13, 513.
Ludwig, T., et al. 1997. Genes Dev 11, 1226.
Ruffner, H. and I.M. Verma. 1997. Proc. Natl. Acad. Sci. USA 94, 7138.
Scully, R., et al. 1997. Cell 90, 425.
Thakur, S., et al. 1997. Mol. Cell Biol. 17, 444.
Wilson, C.A., et al. 1997. Oncogene 14, 1.
Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin Oncol. 23, 1.
Chen, Y., et al. 1996. Cancer Res. 56, 3168.
Liu, C.Y., et al. 1996. Genes Dev. 10, 1835.
Gudas, J.M., et al. 1996. Cell Growth Differ. 7, 717.
Scully, R., et al. 1996. Science 272, 123.
Vaughn, J.P., et al. 1996. Cell Growth Differ. 7, 711.
Wilson, C.A. et al. 1996. Nat. Genet. 13, 264.
Thompson, M.E., et al. 1995. Nat. Genet. 9, 444.
Miki, Y., et al. 1994. Science 266, 66.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
SynonymsAnti-Breast Cancer Susceptibility Protein
ApplicationFrozen Sections (2 µg/ml)
Immunoblotting (2 µg/ml, see application references)
Immunofluorescence (2 µg/ml)
Immunoprecipitation (2 µg, see application references)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing RBF/DnJ mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~220 kDa BRCA1 protein.
BackgroundInactivation of the BRCA1 gene is responsible for some forms of familial breast and ovarian cancer and sporadic ovarian cancer. BRCA1 encodes a protein of 1863 amino acids with an amino-terminal domain that exhibits similarity to the zinc-ring domain found in several regulatory and transcription factors. Two isoforms of BRCA1 are commonly expressed; a full length ~220 kDa protein that is predominantly nuclear and a ~120 kDa form that is missing most of exon 11. Inhibition of BRCA1 expression leads to accelerated growth of mammary cells in vitro while loss of expression is observed during progression from benign disease to metastatic breast cancer. BRCA1 undergoes cell cycle regulation, with mRNA expression induced prior to DNA synthesis, while DNA damage leads to down regulation. BRCA1 protein is also subject to post-translational modification and re-localization at various stages in the cell cycle or in response to DNA damage, and also is required for aspects of transcription-coupled DNA repair. The protein is essential for embryogenesis since mice nullizygous for BRCA1 die in utero, while heterozygosity or conditional knockout of BRCA1 leads to increased tumor formation and aberrant mammary ductal morphology. BRCA1 forms stable associations with a number of proteins including BARD1, p53, BRCA2, Rad51, RNA Pol II holoenzyme, and BAP1. Interestingly, loss of BRCA1 may increase the risk of p53 inactivation, which suggests that BRCA1 is a key element in maintenance of genome integrity.
HostMouse
Immunogen speciesHuman
Immunogena recombinant polypeptide corresponding to amino acids 1005-1313 in exon 11 of human BRCA1
CloneSD118
IsotypeIgG₁
Specieshuman
Positive controlHBL-100 or SK-BR3 cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsMaximal BRCA1 levels are observed in subconfluent cell populations after serum stimulation. Highly specific BRCA1 antibody useful for blotting and IP of full length BRCA1 protein. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesChen, J.J., et al. 1999. Cancer Res. 59, 1752s.
Cressman, V.L., et al. 1999. Cell Growth Differ. 10, 1.
Irminger-Finger, I., et al. 1999. Biol. Chem. 380, 117.
Xu, X., et al. 1999. Nat. Genet. 22, 37.
Wilson, C.A., et al. 1999. Nat. Genet. 21, 236.
Andres, J.L., et al. 1998. Oncogene 16, 2229.
Chen, J., et al. 1998. Mol. Cell 2, 317.
Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 5287.
Fan, S., et al. 1998. Int. J. Cancer 77, 600.
Gowen, L.C., et al. 1998. Science 281, 1009.
Hsu, L.C. and R.L. White. 1998. Proc. Natl. Acad. Sci. USA 95, 12983.
Shen, S.X., et al. 1998. Oncogene 17, 3115.
Tulchin, N., et al. 1998. Int. J. Oncol. 13, 513.
Ludwig, T., et al. 1997. Genes Dev 11, 1226.
Ruffner, H. and I.M. Verma. 1997. Proc. Natl. Acad. Sci. USA 94, 7138.
Scully, R., et al. 1997. Cell 90, 425.
Thakur, S., et al. 1997. Mol. Cell Biol. 17, 444.
Wilson, C.A., et al. 1997. Oncogene 14, 1.
Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin Oncol. 23, 1.
Chen, Y., et al. 1996. Cancer Res. 56, 3168.
Liu, C.Y., et al. 1996. Genes Dev. 10, 1835.
Gudas, J.M., et al. 1996. Cell Growth Differ. 7, 717.
Scully, R., et al. 1996. Science 272, 123.
Vaughn, J.P., et al. 1996. Cell Growth Differ. 7, 711.
Wilson, C.A. et al. 1996. Nat. Genet. 13, 264.
Thompson, M.E., et al. 1995. Nat. Genet. 9, 444.
Miki, Y., et al. 1994. Science 266, 66.
Application referencesImmunoblotting, Immunoprecipitation Wilson, C.A., et al. 1999. Nat. Genet. 21, 236. Original Clone Scully, R., et al. 1996. Science 272, 123.