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OP92 Anti-BRCA1 (Ab-1) Mouse mAb (MS110)

Anti-BRCA1 (Ab-1), mouse monoclonal, clone MS110, recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. It is validated for WB, IF, IP, and frozen and paraffin sections.

Anti-BRCA1 (Ab-1) Mouse mAb (MS110) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Breast Cancer Susceptibility Protein

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OP92
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP92-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not cross-react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
Catalogue NumberOP92
Brand Family Calbiochem®
SynonymsAnti-Breast Cancer Susceptibility Protein
References
ReferencesScully, R., et al. 1996. Science 272, 123.
Gudas, J.M., et al. 1996. Cell Growth Differ. 7, 717.
Vaughn, J.P., et al. 1996. Cell Growth Differ. 7, 711.
Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet. 11, 113.
Merajver, S.D., et al. 1995. Clin. Can. Res. 1, 539.
Merajver, S.D., et al. 1995. Nat. Genet. 9, 439.
Struewing, J.P., et al. 1995. Nat. Genet. 11, 198.
Thompson, M.E., et al. 1995. Nat. Genet. 9, 444.
Futreal, P.A., et al. 1994. Science 266, 120.
Miki, Y., et al. 1994. Science 266, 66.
Wilson, C.A., et al. 1999. Nat. Genet. 21, 236.
Product Information
DeclarationSold under license of U.S. Patents 5,753,441 and 6,162,897.
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlMCF7 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Wilson, C.A., et al. 1999. Nature Genetics 21, 236. Scully, R., et al. 1996. Science 272, 123. Immunoprecipitation, Immunofluorescence Wilson, C.A., et al. 1999. Nature Genetics 21, 236. Scully, R., et al. 1996. Science 272, 123. Frozen Sections, Paraffin Sections Wilson, C.A., et al. 1999. Nature Genetics 21, 236.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (see application reference)
Immunoblotting (2 µg/ml)
Immunofluorescence (see application reference)
Immunoprecipitation (see application reference)
Paraffin Sections (see application reference)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human BRCA1
ImmunogenHuman
Epitopewithin the N-terminal 304 amino acids of BRCA1
CloneMS110
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP92-100UG 04055977209686

Documentation

Anti-BRCA1 (Ab-1) Mouse mAb (MS110) Certificates of Analysis

TitleLot Number
OP92

References

Reference overview
Scully, R., et al. 1996. Science 272, 123.
Gudas, J.M., et al. 1996. Cell Growth Differ. 7, 717.
Vaughn, J.P., et al. 1996. Cell Growth Differ. 7, 711.
Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet. 11, 113.
Merajver, S.D., et al. 1995. Clin. Can. Res. 1, 539.
Merajver, S.D., et al. 1995. Nat. Genet. 9, 439.
Struewing, J.P., et al. 1995. Nat. Genet. 11, 198.
Thompson, M.E., et al. 1995. Nat. Genet. 9, 444.
Futreal, P.A., et al. 1994. Science 266, 120.
Miki, Y., et al. 1994. Science 266, 66.
Wilson, C.A., et al. 1999. Nat. Genet. 21, 236.

Citations

Title
  • Richard D. Kennedy, et al. (2005) BRCA1 and c-Myc associate to transcriptionally repress psoriasin, a DNA damage-inducible gene. Cancer Research 65, 10265-10272.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision03-October-2007 RFH
    SynonymsAnti-Breast Cancer Susceptibility Protein
    ApplicationFrozen Sections (see application reference)
    Immunoblotting (2 µg/ml)
    Immunofluorescence (see application reference)
    Immunoprecipitation (see application reference)
    Paraffin Sections (see application reference)
    DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.
    BackgroundInactivation of the BRCA1 gene, located at 17q21, is responsible for some forms of familial breast and ovarian cancer and sporadic ovarian cancer. BRCA1 encodes a protein of 1863 amino acids with an amino-terminal domain that exhibits similarity to the zinc-ring domain found in several regulatory and transcription factors. Mutations that disrupt this domain are some of the most frequently occurring ones linked to breast and ovarian cancer. Inhibition of BRCA1 expression leads to accelerated growth of mammary cells in vitro while loss of expression is observed during progression from benign disease to metastatic breast cancer suggesting that BRCA1 serves as a negative growth regulator. The ~220 kDa BRCA1 protein is expressed predominantly in the nucleus and appears to undergo cell cycle regulation with mRNA expression induced prior to DNA synthesis.
    HostMouse
    Immunogen speciesHuman
    Immunogenrecombinant human BRCA1
    Epitopewithin the N-terminal 304 amino acids of BRCA1
    CloneMS110
    IsotypeIgG₁
    Specieshuman
    Positive controlMCF7 cells
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAntibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesScully, R., et al. 1996. Science 272, 123.
    Gudas, J.M., et al. 1996. Cell Growth Differ. 7, 717.
    Vaughn, J.P., et al. 1996. Cell Growth Differ. 7, 711.
    Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet. 11, 113.
    Merajver, S.D., et al. 1995. Clin. Can. Res. 1, 539.
    Merajver, S.D., et al. 1995. Nat. Genet. 9, 439.
    Struewing, J.P., et al. 1995. Nat. Genet. 11, 198.
    Thompson, M.E., et al. 1995. Nat. Genet. 9, 444.
    Futreal, P.A., et al. 1994. Science 266, 120.
    Miki, Y., et al. 1994. Science 266, 66.
    Wilson, C.A., et al. 1999. Nat. Genet. 21, 236.
    Citation
  • Richard D. Kennedy, et al. (2005) BRCA1 and c-Myc associate to transcriptionally repress psoriasin, a DNA damage-inducible gene. Cancer Research 65, 10265-10272.
    		•
    Application referencesImmunoblotting Wilson, C.A., et al. 1999. Nature Genetics 21, 236. Scully, R., et al. 1996. Science 272, 123. Immunoprecipitation, Immunofluorescence Wilson, C.A., et al. 1999. Nature Genetics 21, 236. Scully, R., et al. 1996. Science 272, 123. Frozen Sections, Paraffin Sections Wilson, C.A., et al. 1999. Nature Genetics 21, 236.