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MABF3094-100UL Anti-BAFF/CD257 Antibody, clone 5A8

Anti-BAFF/CD257, clone 5A8, Cat. No. MABF3094, is a rat monoclonal antibody that detects BAFF/CD257 and is tested for use in Activity Assay, ELISA, and Western Blotting,

MABF3094-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3094-100UL
DescriptionAnti-BAFF/CD257 Antibody, clone 5A8
Alternate Names
  • Tumor necrosis factor ligand superfamily member 13B
  • B-cell-activating factor
  • BAFF
  • CD257
Background InformationTumor necrosis factor ligand superfamily member 13B (UniProt: Q9WU72; also known as B-cell-activating factor, BAFF, CD257) is encoded by the Tnfsf13b (also known as Baff) gene (Gene ID: 24099) in murine species. BAFF is a single-pass type II, homotrimeric, membrane glycoprotein with a cytoplasmic domain (aa 1-47), a transmembrane domain (aa 48-68), and an extracellular domain (aa 69-309). A soluble form of BAFF has also been described that is produced by the cleavage of membrane form between amino acids 126-127. It is expressed by cells of myeloid origin and by stromal cells. Two isoforms of BAFF have been described that are produced by alternative splicing. Isoform 2 is reported to inhibit the secretion and bioactivity of isoform 1. BAFF is reported to bind to TNFRSF13B/TACI and TNFRSF17/BCMA. It serves as a potent B cell survival factor and is a critical immune checkpoint during peripheral B cell maturation. Its levels are often elevated in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Elevated levels of BAFF are also observed in subjects with non-Hodgkin s lymphoma (NHL) and it is reported to promote survival of both NHL-derived B lymphoma cells and B cell chronic lymphocytic leukemia (B-CLL) cells. (Ref.: Vigolo, M., et al. (2018). Nat. Commun. 9(1); 1199; Kowalczyk-Quintas, C., et al (2016). J. Biol. Chem. 291(38); 19826-19834; Batten, M., et al. (2004). J. Immunol. 172(2); 812-822; Gavin, AL., et al. (2003). J. Biol. Chem. 278(40); 38220-38228).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-BAFF/CD257, clone 5A8, Cat. No. MABF3094, is a rat monoclonal antibody that detects BAFF/CD257 and is tested for use in Activity Assay, ELISA, and Western Blotting,
Key Applications
  • Western Blotting
  • ELISA
  • Activity Assay
Application NotesTested applications

Activity Assay: A representative lot activated mouse BAFF in a reporter cell assay, in primary B cells. (Kowalczyk-Quintas, C., et al. (2016). J Biol.Chem.291(38):19826-34; Vigolo, M., et al. (2018). Nat Commun.9(1):1199).

ELISA Analysis: A representative lot detected BAFF/CD257 in ELISA applications (Batten, M., et al. (2004). J Immunol. 172(2):812-22; Vigolo, M., et al. (2018). Nat Commun. 9(1):1199; Kowalczyk-Quintas, C., et al. (2016). J Biol Chem. 291(38):19826-34).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRat RBL cells expressing full-length mouse BAFF.
EpitopeUnknown
Clone5A8
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 5A8 is a rat monoclonal antibody that detects B-cell activating factor (BAFF/CD257).
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Klrk1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~ 20 and 43 kDa observed; 34.19 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant murine BAFF protein .

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant murine BAFF/CD257 protein .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3094-100UL 04065269125914