Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN1831 Anti-Atlastin-1 Antibody, clone 3194

Anti-Atlastin-1 Antibody, clone 3194 is an antibody against Atlastin-1 for use in Western Blotting, Immunocytochemistry.

Anti-Atlastin-1 Antibody, clone 3194 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: ATL1, GBP3, HSN1D, SPG3, SPG3A UniProt Number: Q8WXF7
View Products on Sigmaaldrich.com
MABN1831
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1831
DescriptionAnti-Atlastin-1 Antibody, clone 3194
Alternate Names
  • Atlastin-1
  • Brain-specific GTP-binding protein
  • GBP-3
  • GTP-binding protein 3
  • Guanine nucleotide-binding protein 3
  • hGBP3
  • Spastic paraplegia 3 protein A
Background InformationAtlastin-1 (UniProt Q8WXF7; also known as Brain-specific GTP-binding protein, GBP-3, GTP-binding protein 3, Guanine nucleotide-binding protein 3, hGBP3, Spastic paraplegia 3 protein A) is encoded by the ATL1 (also known as GBP3, HSN1D, SPG3, SPG3A) gene (Gene ID 51062) in human. Atlastin-1 is a dynamin/Mx/guanylate-binding protein superfamily member with three conserved guanylate-binding/GTPase active site motifs, P-loop (a.a. 74-81), DxxG (a.a. 146-149), and RD (217-218). Atlastin-1 is a multimeric integral membrane protein localized on ER and cis-Golgi apparatus and widely expressed in many tissues, including the smooth muscle, adrenal gland, kidney, testis, lung, and brain. Atlastin-1 interacts with the microtubule-severing ATPase spastin as well as with the DP1/Yop1p and reticulon families of ER-shaping proteins, Atlastin-1 knockdown results in decreased number of neuronal processes and impaired axon formation in cultured cortical neurons, while atlastin-1 overexpression increases total dendrite length both in vivo and in vitro. ALT1 gene mutations cause abnormal ER morphology and are linked to hereditary spastic paraplegias (HSPs), a genetically heterogeneous group of neurological disorders, includiing SPG3 (spastic paraplegia 3, autosomal dominan) and HSN1D (neuropathy, hereditary sensory, 1D), characterized by lower limb spasticity and weakness. Human atlastin-1 is a 492-amino acid protein that passes through the ER membrane twice (a.a. 450-470, 472-492), having both its N- and C-terminal ends at the cytoplasmic side (a.a. 1-449, 493-558).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Atlastin-1 Antibody, clone 3194 is an antibody against Atlastin-1 for use in Western Blotting, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 10 µg/mL from a representative lot detected Atlastin-1 in 10 µg of mouse brain tissue lysate.
Immunocytochemistry Analysis: A representative lot localized Atlastin-1 immunoreactivity with that of REEP1 by dual fluorescent immunocytochemistry staining of paraformaldehyde-fixed rat cortical neurons (Park, S.H., et al. (2010). J. Clin. Invest. 120(4):1097-1110).
Immunocytochemistry Analysis: A representative lot localized overexpressed human Atlastin-1 in paraformaldehyde-fixed COS7 transfectants by fluorescent immunocytochemistry (Park, S.H., et al. (2010). J. Clin. Invest. 120(4):1097-1110).
Biological Information
ImmunogenLinear peptide corresponding to the N-terminal sequence of human Atlastin-1.
EpitopeNear N-terminus.
Clone3194
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3194 targets an N-terminal epitope present in both spliced isoforms of human Atlastin-1 reported by UniProt (Q8WXF7).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Monkey based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATL1
  • GBP3
  • HSN1D
  • SPG3
  • SPG3A
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~63 kDa observed. 63.54/63.06 (human isoform 1/2), 63.52 kDa (monkey), and 63.38 kDa (mouse and rat) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Atlastin-1 in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1831 04055977350142