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AB3152 Anti-Amphiregulin Antibody

This Anti-Amphiregulin Antibody is validated for use in ELISA, IP, WB for the detection of Amphiregulin.

Anti-Amphiregulin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurochemistry & Neurotrophins Gene Symbol: AREG, MGC13647, CRDGF, amphiregulin, AR, SDGF UniProt Number: P15514
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AB3152
100 µg  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MELISA, IP, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB3152
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Amphiregulin Antibody
Alternate Names
  • AR
  • CRDGF
  • KAF
References
Product Information
FormatPurified
PresentationPurified immunoglobulin from rabbit anti-serum by Protein A chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Amphiregulin Antibody is validated for use in ELISA, IP, WB for the detection of Amphiregulin.
Key Applications
  • ELISA
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation (Use Protein A): Ab 10μg/mg protein lysate.

Western Blotting: Ab 5μg/ml for 2hrs at room temperature.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenA synthetic 19-mer peptide, corresponding to aa 26-44 from the secreted amphiregulin of human origin.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes a glycoprotein of 18.5-22.5kDa, identified as amphiregulin (AR). Antibody is directed against the region of AR, which binds to heparin and is highly specific to AR. It shows no cross-reaction with EGF, TGFalpha, or HB-EGF. In vitro, AR functions as an autocrine growth stimulator to derive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. AR is important in development of human placenta and murine mammary gland.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype.
Gene Symbol
  • AREG
  • MGC13647
  • CRDGF
  • amphiregulin
  • AR
  • SDGF
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P15514 # Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
SIZE: 252 amino acids; 27895 Da
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein.
SIMILARITY: SwissProt: P15514 ## Belongs to the amphiregulin family. & Contains 1 EGF-like domain.
MISCELLANEOUS: AR is a protein containing cysteines in disulfide linkage(s) that are essential for its biological activity. AR may contain oligosaccharides and/or lipid moieties that are not obligatory for the biological activity.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB3152 04053252731754

Documentation

Anti-Amphiregulin Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Amphiregulin Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-AMPHIREGULIN 2830926
RABBIT ANTI-AMPHIREGULIN - 3321167 3321167
RABBIT ANTI-AMPHIREGULIN - 3835393 3835393
RABBIT ANTI-AMPHIREGULIN - 4116921 4116921
RABBIT ANTI-AMPHIREGULIN -2851253 2851253
RABBIT ANTI-AMPHIREGULIN POLYCLONAL ANTIBODY 3079146
RABBIT ANTI-AMPHIREGULIN POLYCLONAL ANTIBODY 2935538
RABBIT ANTI-AMPHIREGULIN POLYCLONAL ANTIBODY 3036930
RABBIT ANTI-AMPHIREGULIN POLYCLONAL ANTIBODY - 2080269 2080269
RABBIT ANTI-AMPHIREGULIN POLYCLONAL ANTIBODY -2582839 2582839
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References

Reference overviewPub Med ID
Structure and function of human amphiregulin: a member of the epidermal growth factor family
Shoyab, M, et al
Science, 243:1074-1076 (1989)  1989

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