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PC522 Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb

Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11), rabbit polyclonal, recognizes ~35 kDa ANT-1 in doxorubicin-treated MCF-7 cells. It is validated for WB, immunofluorescence, and paraffin sections.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ANT-1

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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
OverviewRecognizes the ~35 kDa ANT-1 protein in doxorubicin-treated MCF-7 cells or breast carcinoma tissue.
  • Antibody Target Gene Symbol: ANK3
  • Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
  • Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
  • Hu Entrez ID: 288 (Related Antibodies: NB20, AP1034, PC523, AP1057)
  • Mu Entrez ID: 11735
  • Rat Entrez ID: 361833
    		•
    Catalogue NumberPC522
    Brand Family Calbiochem®
    SynonymsAnti-ANT-1
    Application Data
    Detection of human ANT-1 by staining paraffin sections. Sample: Breast carcinoma. Primary antibody: Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb (Cat. No. PC522) (1 µg/ml). Detection: DAB with methyl green counterstain.
    References
    ReferencesBauer, M.K.A., et al. 1999. J. Cell Biol. 147, 1493.
    Marzo, I, et al. 1998. Science 281, 2027.
    Marzo, I, et al. 1998. J. Exp. Med. 187, 1261.
    Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta 1231, 139.
    Klingenberg, M. 1993. J. Bioenerg. Biomembr. 25, 447.
    Riccio, P., et al. 1975. FEBS Lett. 56, 133.
    Product Information
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
    Positive controldoxorubicin-treated MCF7 cells, or breast carcinoma tissue
    PreservativeNone
    Applications
    Key Applications Immunoblotting (Western Blotting)
    Immunofluorescence
    Paraffin Sections
    Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence)
    Immunofluorescence (1 µg/ml)
    Paraffin Sections (1 µg/ml, heat pre-treatment required)
    Application CommentsMCF7 cells treated with doxorubicin at 0.2 µg/ml for 24 h can be used as positive control for immunoblotting. Antibody should be titrated for optimal results in individual systems.
    Biological Information
    Immunogena synthetic peptide corresponding to amino acids 2-11 of human ANT-1
    ImmunogenHuman
    HostRabbit
    IsotypeIgG
    Species Reactivity
    • Human
    Antibody TypePolyclonal Antibody
    Concentration Label Please refer to vial label for lot-specific concentration
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Dry Ice Only
    Toxicity Standard Handling
    Storage -20°C
    Avoid freeze/thaw Avoid freeze/thaw
    Do not freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    Catalogue Number GTIN
    PC522 0

    Documentation

    Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb SDS

    Title

    Safety Data Sheet (SDS) 

    Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb Certificates of Analysis

    TitleLot Number
    PC522

    References

    Reference overview
    Bauer, M.K.A., et al. 1999. J. Cell Biol. 147, 1493.
    Marzo, I, et al. 1998. Science 281, 2027.
    Marzo, I, et al. 1998. J. Exp. Med. 187, 1261.
    Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta 1231, 139.
    Klingenberg, M. 1993. J. Bioenerg. Biomembr. 25, 447.
    Riccio, P., et al. 1975. FEBS Lett. 56, 133.
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision29-June-2007 RFH
    SynonymsAnti-ANT-1
    ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence)
    Immunofluorescence (1 µg/ml)
    Paraffin Sections (1 µg/ml, heat pre-treatment required)
    Application Data
    Detection of human ANT-1 by staining paraffin sections. Sample: Breast carcinoma. Primary antibody: Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb (Cat. No. PC522) (1 µg/ml). Detection: DAB with methyl green counterstain.
    DescriptionPurified rabbit polyclonal antibody. Recognizes the ~35 kDa ANT-1 protein.
    BackgroundANT-1 (adenine nucleotide translocase-1) (~35 kDa) is a key component of the mitochondrial permeability transition pore (PT pore) complex and is also one of the most abundant proteins in the mitochondrion making up approximately 10% of all mitochondrial proteins. Originally described as an ADP/ATP exchange factor, ANT-1 has also been shown to be required for bax mediated apoptosis. This is effected by binding of bax to residues 105-156 of ANT-1, presumably resulting in maintaining the PT pore in an open conformation. Interaction with bax is not required for ANT-1 pro-apoptotic activity as overexpression of the ANT-1 protein also leads to the induction of apoptosis.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide corresponding to amino acids 2-11 of human ANT-1
    IsotypeIgG
    Specieshuman
    Positive controldoxorubicin-treated MCF7 cells, or breast carcinoma tissue
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
    Concentration Label Please refer to vial label for lot-specific concentration
    PreservativeNone
    CommentsMCF7 cells treated with doxorubicin at 0.2 µg/ml for 24 h can be used as positive control for immunoblotting. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesBauer, M.K.A., et al. 1999. J. Cell Biol. 147, 1493.
    Marzo, I, et al. 1998. Science 281, 2027.
    Marzo, I, et al. 1998. J. Exp. Med. 187, 1261.
    Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta 1231, 139.
    Klingenberg, M. 1993. J. Bioenerg. Biomembr. 25, 447.
    Riccio, P., et al. 1975. FEBS Lett. 56, 133.