Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN852-100UG Anti-APP Antibody, clone 2B3

Anti-APP, clone 2B3, Cat. No. MABN852, is a highly specific mouse monoclonal antibody that targets Amyloid beta A4 protein and has been tested for use in ELISA, Immunocytochemistry, Inhibiton Assay, and Western Blotting.

Anti-APP Antibody, clone 2B3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: APP, A4, AD1 UniProt Number: P05067
View Products on Sigmaaldrich.com
MABN852-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HELISA, ICC, Inhibition, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN852-100UG
DescriptionAnti-APP Antibody, clone 2B3
Alternate Names
  • Amyloid-beta A4 protein
  • ABPP
  • APPI
  • Alzheimer disease amyloid protein
  • Amyloid precursor protein
  • Amyloid-beta precursor protein
  • Cerebral vascular amyloid peptide
  • CVAP
  • PreA4
  • Protease nexin-II
  • PN-II
Background InformationAmyloid-beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimer's disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
Applications
ApplicationAnti-APP, clone 2B3, Cat. No. MABN852, is a highly specific mouse monoclonal antibody that targets Amyloid beta A4 protein and has been tested for use in ELISA, Immunocytochemistry, Inhibiton Assay, and Western Blotting.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Inhibition
  • Western Blotting
Application NotesELISA Analysis: A representative lot detected APP in ELISA applications (Thomas, R.S., et. al. (2011). FEBS J. 278(1):167-78; Thomas, R.S., et. al. (2013). Neuroreport. 24(18):1058-61).

Inhibition Analysis: A representative lot inhibited beta amyloid production in MOG-G-UVW culture media (Thomas, R.S., et. al. (2011). FEBS J. 278(1):167-78).

Western Blotting Analysis: A representative lot detected APP in Western Blotting applications (Thomas, R.S., et. al. (2011). FEBS J. 278(1):167-78).

Immunocytochemistry Analysis: A representative lot detected APP in Immunocytochemistry applications (Thomas, R.S., et. al. (2013). Neuroreport. 24(18):1058-61).
Biological Information
ImmunogenA linear peptide corresponding to 15 amino acids from the C-terminal region of human Amyloid-beta A4 protein.
Epitopeextracellular domain
Clone2B3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2B3 specifically detects Amyloid-beta A4 protein in human and murine neurons. It targets an epitope within 15 amino acids including the beta-secretase cleavage site.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APP
  • A4
  • AD1
Purification MethodProtein G purified
UniProt Number
Molecular Weight86.94 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in mouse cortical neurons.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected APP in mouse cortical neurons.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN852-100UG 04054839460197