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OP80 Anti-APC (Ab-7) Mouse mAb (CC-1)

Anti-APC (Ab-7), mouse monoclonal, clone CC-1, recognizes APC in oligodendrocytes and astrocytes. It is validated for ICC, IF, free-floating Sections, and paraffin and frozen sections.

Anti-APC (Ab-7) Mouse mAb (CC-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Adenomatus Polyposis Coli

OP80
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP80-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes APC in oligodendrocytes and astrocytes.
  • Antibody Target Gene Symbol: APC
  • Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
  • Entrez Gene Name: adenomatous polyposis coli
  • Hu Entrez ID: 324 (Related Antibodies: ST1150, OP62, OP47L, OP44)
  • Mu Entrez ID: 11789
  • Rat Entrez ID: 24205
    		•
    Catalogue NumberOP80
    Brand Family Calbiochem®
    SynonymsAnti-Adenomatus Polyposis Coli
    References
    ReferencesBhat, R.V., et al. 1996. Glia 17, 169.
    Bhat, R.V., et al. 1994. Neuroscience Proceedings.
    Smith, K.J., et al. 1994. Cancer Res. 54, 3672.
    Smith, K.J., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2846.
    Su, L.-K., et al. 1993. Cancer Res. 53, 2728.
    Su, L.-K., et al. 1993. Science 262, 1734.
    Groden, J., et al. 1991. Cell 66, 589.
    Kinzler, K.W., et al. 1991. Science 251, 1366.
    Product Information
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
    Negative controlMOPC 21 cells
    Positive controlPurkinje cells or cerebellum tissue
    Preservative≤0.1% sodium azide
    Quality LevelMQ100
    Applications
    Application ReferencesImmunocytochemistry McTigue, D.M., et al. 2001. J. Neurosci. 21, 3392. Free Floating Sections Ding, H., et al. 2003. Cancer Res. 63, 1106.
    Key Applications Free-floating Sections
    Frozen Sections
    Immunocytochemistry
    Immunofluorescence
    Paraffin Sections
    Application NotesFrozen Sections (5 µg/ml)
    Immunofluorescence (5 µg/ml)
    Paraffin Sections (5 µg/ml, heat pre-treament required)
    Immunocytochemistry (see application references)
    Free Floating Sections (see application references)
    Application CommentsWell suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody's staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.
    Biological Information
    Immunogena recombinant protein consisting of amino acids 1-226 of APC
    CloneCC-1
    HostMouse
    IsotypeIgG2b
    Species Reactivity
    • Human
    • Mouse
    • Rat
    Antibody TypeMonoclonal Antibody
    Concentration Label Please refer to vial label for lot-specific concentration
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Blue Ice Only
    Toxicity Standard Handling
    Storage +2°C to +8°C
    Do not freeze Yes
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    Catalogue Number GTIN
    OP80-100UG 04055977209662

    Documentation

    Anti-APC (Ab-7) Mouse mAb (CC-1) Certificates of Analysis

    TitleLot Number
    OP80

    References

    Reference overview
    Bhat, R.V., et al. 1996. Glia 17, 169.
    Bhat, R.V., et al. 1994. Neuroscience Proceedings.
    Smith, K.J., et al. 1994. Cancer Res. 54, 3672.
    Smith, K.J., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2846.
    Su, L.-K., et al. 1993. Cancer Res. 53, 2728.
    Su, L.-K., et al. 1993. Science 262, 1734.
    Groden, J., et al. 1991. Cell 66, 589.
    Kinzler, K.W., et al. 1991. Science 251, 1366.
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision16-November-2007 JSW
    SynonymsAnti-Adenomatus Polyposis Coli
    ApplicationFrozen Sections (5 µg/ml)
    Immunofluorescence (5 µg/ml)
    Paraffin Sections (5 µg/ml, heat pre-treament required)
    Immunocytochemistry (see application references)
    Free Floating Sections (see application references)
    DescriptionPurified mouse monoclonal antibody generated by immunizing CB6 F1 mice with the specified immunogen and fusing splonocytes with SP2/O mouse myeloma cells. Recognizes the ~312 kDa APC protein.
    BackgroundGerm line mutations in the APC gene are responsible for familial adenomatous polyposis and somatic mutations contribute to sporadic colorectal cancer. The 312 kDa APC protein is expressed in all tissues and localized to the cytoskeletal architecture in an insoluble complex with β-catenin. The APC (Ab-7) mouse monoclonal antibody clone CC-1 decorates oligodendrocyte cell bodies, purkinje cells, astrocytes, and optic nerve cell bodies of formalin-fixed, paraffin-embedded, or fresh frozen sections from human, mouse, and rat brain. Dectection of APC (Ab-7) can be perfomed using immunofluorescence or immunohistochemistry techniques.
    HostMouse
    Immunogena recombinant protein consisting of amino acids 1-226 of APC
    CloneCC-1
    IsotypeIgG2b
    Specieshuman, mouse, rat
    Positive controlPurkinje cells or cerebellum tissue
    Negative controlMOPC 21 cells
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsWell suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody's staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesBhat, R.V., et al. 1996. Glia 17, 169.
    Bhat, R.V., et al. 1994. Neuroscience Proceedings.
    Smith, K.J., et al. 1994. Cancer Res. 54, 3672.
    Smith, K.J., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2846.
    Su, L.-K., et al. 1993. Cancer Res. 53, 2728.
    Su, L.-K., et al. 1993. Science 262, 1734.
    Groden, J., et al. 1991. Cell 66, 589.
    Kinzler, K.W., et al. 1991. Science 251, 1366.
    Application referencesImmunocytochemistry McTigue, D.M., et al. 2001. J. Neurosci. 21, 3392. Free Floating Sections Ding, H., et al. 2003. Cancer Res. 63, 1106.