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MABE1790-25UG Anti-ADAR1 Antibody, clone RD4B11

Anti-ADAR1, clone RD4B11, Cat. No. MABE1790, is a highly specific rat monoclonal antibody that targets ADAR1 and has been tested for use in Western Blotting.

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MABE1790-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABE1790-25UG
DescriptionAnti-ADAR1 Antibody, clone RD4B11
Alternate Names
  • Double-stranded RNA-specific adenosine deaminase
  • EC: 3.5.4.37
  • DRADA
  • RNA adenosine deaminase 1
Background InformationDouble-stranded RNA-specific adenosine deaminase (UniProt: Q99MU3; also known as EC: 3.5.4.37, DRADA, RNA adenosine deaminase 1) is encoded by the Adar gene (Gene ID: 56417) in murine species. ADAR catalyzes the editing of adenosine-to-inosine (A to I) by deaminating genomically encoded A-to-I in double stranded RNA (DsRNA). A-to-I editing predominantly occurs in noncoding, repetitive elements and short interspersed nuclear elements (SINEs). A-to-I editing affects gene expression and function in several ways, including mRNA translation by changing codons and hence the amino acid sequence of proteins. It also affects pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; and genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication. In mammals three different ADAR proteins have been described: ADAR1, -2, and -3. ADAR1 is shown to be expressed during embryonic and postnatal development and is present predominantly in the nucleus. Highest levels of ADAR1 are reported in brain and spleen and lowest levels are observed in liver. Five different isoforms of ADAR1 have been reported that are produced by alternative splicing. Isoforms 1 and 2 (long forms) are predominantly found in cytoplasm and shuttle between the cytoplasm and nucleus. Isoforms 3 and 4 (short forms starting at Met-519) are exclusively found in the nucleolus. Under normal conditions long forms are dominant, however, under conditions of inflammation short forms are selectively induced. (Ref.: Liddicoat, BJ et al., (2015). Science 349 (6252); 1115-1120).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-ADAR1, clone RD4B11, Cat. No. MABE1790, is a highly specific rat monoclonal antibody that targets ADAR1 and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected ADAR1 in Western Blotting applications (Liddicoat, B.J., et. al. (2015). Science. 349(6252):1115-20).
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 401 amino acids from the internal region of murine Double-stranded RNA-specific adenosine deaminase (ADAR1). Immunogen sequnece is conserved isoforms 1 and 2.
CloneRD4B11
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone RD4B11 is a rat monoclonal antibody that detects Double-stranded RNA-specific adenosine deaminase in human and murine cells.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Adar
Purification MethodProtein G purified
UniProt Number
Molecular Weight~110 kDa observed; 130.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Kusa4b10 mouse cell line overexpressing mouse ADAR1 p110.

Western Blotting Analysis: 4 µg/mL of this antibody detected ADAR1 in Kusa4b10 mouse cell line overexpressing mouse ADAR1 p110.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1790-25UG 04054839499586

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