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PC528 Anti-ADAM10 (Ab-1) (732-748) Rabbit pAb

Anti-ADAM10 (Ab-1) (732-748) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Kuz

PC528
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.



Please refer to our complete listing of ADAM10 antibodies for possible alternatives.






Recognizes the ~85 kDa ADAM10 precursor, a faint 60 kDa band corresponding to processed mature protein, and a 45 kDa degradation product in Jurkat cells.
  • Antibody Target Gene Symbol: ADAM10
  • Target Synonym: 1700031C13Rik, AD10, ALPHA SECRETASE, CD156c, DISINTEGRIN-METALLOPROTEASE, HsT18717, Kuz, KUZBANIAN, MADM
  • Entrez Gene Name: ADAM metallopeptidase domain 10
  • Hu Entrez ID: 102 (Related Antibodies: 422751)
  • Mu Entrez ID: 11487
  • Rat Entrez ID: 29650
    		•
    Catalogue NumberPC528
    Brand Family Calbiochem®
    SynonymsAnti-Kuz
    Application Data
    Detection of human ADAM10 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ADAM10 (Ab-1) Rabbit pAb (Cat. No. PC528) (1:1000) pre-incubated without (lane 1) or with (lane 2) blocking peptide. Detection: chemiluminescence
    References
    ReferencesQi, H., et al. 1999. Science 283, 91.
    Pan, D. and Kuzbanian, G.M. 1997. Cell 90, 271.
    Rosendahl, M.S., et al. 1997. J. Biol. Chem. 274, 24588.
    Howard, L., et al. 1996. Biochem. J. 317, 45.
    Product Information
    FormLiquid
    FormulationIn PBS.
    Positive controlJurkat cell lysate
    Preservative≤0.1% sodium azide
    Applications
    Key Applications Immunoblotting (Western Blotting)
    Application NotesImmunoblotting (1:500-1:2000)
    Application CommentsAntibody should be titrated for optimal results in individual systems.
    Biological Information
    Immunogena synthetic peptide (PQRQRPRESYQMGHMRR) corresponding to amino acids 732-748 of human ADAM10
    ImmunogenHuman
    HostRabbit
    IsotypeIgG
    Concentration Label Please refer to vial label for lot-specific concentration
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Dry Ice Only
    Toxicity Standard Handling
    Storage -20°C
    Avoid freeze/thaw Avoid freeze/thaw
    Do not freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freze (-20°C).
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    Catalogue Number GTIN
    PC528 0

    Documentation

    Anti-ADAM10 (Ab-1) (732-748) Rabbit pAb Certificates of Analysis

    TitleLot Number
    PC528

    References

    Reference overview
    Qi, H., et al. 1999. Science 283, 91.
    Pan, D. and Kuzbanian, G.M. 1997. Cell 90, 271.
    Rosendahl, M.S., et al. 1997. J. Biol. Chem. 274, 24588.
    Howard, L., et al. 1996. Biochem. J. 317, 45.

    Citations

    Title
  • Daniel W. Lambert, et al. (2005) Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the SARS-CoV receptor, angiotensin-converting enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
  • Daniel W. Lambert, et al. (2005) Tumor Necrosis Factor- Convertase (ADAM17) Mediates Regulated Ectodomain Shedding of the Severe-acute Respiratory Syndrome-Coronavirus (SARS-CoV) Receptor, Angiotensin-converting Enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision11-October-2007 RFH
    SynonymsAnti-Kuz
    ApplicationImmunoblotting (1:500-1:2000)
    Application Data
    Detection of human ADAM10 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ADAM10 (Ab-1) Rabbit pAb (Cat. No. PC528) (1:1000) pre-incubated without (lane 1) or with (lane 2) blocking peptide. Detection: chemiluminescence
    DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~85 kDa ADAM10 protein.
    BackgroundProinflammatory cytokine tumor necrosis factor-α (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including T-cell lymphoma. TNF-α and its ligand delta are all membrane-bound molecules, which are cleaved by proteases to release mature proteins or functional receptors. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM, was recently identified to cleave TNF-α, notch, and it's ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10, an ~85 kDa protein, is expressed in a variety of human and bovine tissues.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide (PQRQRPRESYQMGHMRR) corresponding to amino acids 732-748 of human ADAM10
    IsotypeIgG
    Specieshuman
    Positive controlJurkat cell lysate
    FormLiquid
    FormulationIn PBS.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAntibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freze (-20°C).
    Toxicity Standard Handling
    ReferencesQi, H., et al. 1999. Science 283, 91.
    Pan, D. and Kuzbanian, G.M. 1997. Cell 90, 271.
    Rosendahl, M.S., et al. 1997. J. Biol. Chem. 274, 24588.
    Howard, L., et al. 1996. Biochem. J. 317, 45.
    Citation
  • Daniel W. Lambert, et al. (2005) Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the SARS-CoV receptor, angiotensin-converting enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
  • Daniel W. Lambert, et al. (2005) Tumor Necrosis Factor- Convertase (ADAM17) Mediates Regulated Ectodomain Shedding of the Severe-acute Respiratory Syndrome-Coronavirus (SARS-CoV) Receptor, Angiotensin-converting Enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
    		•