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MABF2771-100UL Anti-564/idiotype Antibody, clone 9D11

Anti-564/idiotype, clone 9D11, Cat. No. MABF2771, is a mouse monoclonal antibody that detects mouse IgG idiotype and is tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, , Immunoprecipitation, & Western Blotting.

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MABF2771-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABF2771-100UL
DescriptionAnti-564/idiotype Antibody, clone 9D11
Alternate Names
  • Idiotype
Background InformationAn idiotype is the genetically determined variation of structures in the variable regions of immunoglobulins. Anti-idiotypic antibodies are known to target specific epitopes, on another antibody that are unique for that target antibody. The collection of epitopes for a given antibody defines its idiotype and is generally found in the complementarity determining regions of that targeted antibody. These antibodies can also bind shared epitopes on highly related monoclonal antibodies. Their specificity allows for the specific detection of antibody of interest even in the presence of endogenous polyclonal antibody. Due to their specificity for the variable region, these antibodies can also be useful in pharmacokinetic and immunogenicity studies. 564Igi is shown to be a reliable murine model of systemic lupus erythematous (SLE). These mice develop SLE profile closely resembling that of human SLE. These mice possess rearranged immunoglobulin heavy- and light-chain genes inserted into their respective loci that are together specific for nucleolar antigens. The 564 antibody is reported to have a characteristic idiotype and B cells carrying the corresponding B-cell antigen receptor are idiotype positive. The epitope recognized by the 564 idiotype antibody is a domain that may be common to multiple self-antigens. (Ref.: Berland, R., et al. (2006). Immunity 25(3); 429-440).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-564/idiotype, clone 9D11, Cat. No. MABF2771, is a mouse monoclonal antibody that detects mouse IgG idiotype and is tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, , Immunoprecipitation, & Western Blotting.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesTested Applications

Flow Cytometry Analysis: A representative lot detected 564/idiotype in Flow Cytometry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450; van der Poel, C.E., et al. (2019). Cell Rep. 29(9):2745-2755.e4; Simoni, L., et al. (2020). Cell Rep. 33(5):108330).

Immunohistochemistry Applications: A representative lot detected 564/idiotype in Immunohistochemistry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

Immunoprecipitation Analysis: A representative lot detected 564/idiotype in Immunoprecipitation applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

Immunocytochemistry Analysis: A representative lot detected 564/idiotype in Immunocytochemistry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

Immunofluorescence Analysis: A representative lot detected 564/idiotype in Immunofluorescence applications (Simoni, L., et al. (2020). Cell Rep. 33(5):108330).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenAn idiotype of a mouse IgG (clone C11) representing B cell receptor expressed by auto reactive B cells in 564 Igi mice.
EpitopeUnknown
Clone9D11
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 9D11 is a mouse monoclonal antibody that detects idiotype of a mouse IgG.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G purified
UniProt Number
Molecular Weight~54 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Idiotype of a mouse IgG target antigen (clone C11).

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected idiotype of a mouse IgG target antigen (clone C11) .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2771-100UL 04065267613987

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