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MABE251 Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31

Detect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology
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MABE251
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
ADB, Methylated DNA Immunoprecipitation (MeDIP), ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE251
DescriptionAnti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31
Background Information5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.
References
Product Information
FormatPurified
Control
  • Unmodified and modified cytosine DNA.
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.
Key Applications
  • Dot Blot
  • Methylated DNA Immunoprecipitation (MeDIP)
  • ELISA
Application NotesELISA Analysis: A representative lot detected 5-hydroxymethylcytosine in ELISA.

Methylated DNA Immunoprecipitation (MeDIP) Analysis: A representative lot immunoprecipitated 5-hydroxymethylcytosine in ChIP.
Biological Information
ImmunogenBSA-conjugated 5-hydroxymethylcytosine.
Epitope5-hydroxymethylcytosine
CloneHMC 31
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the 5-hydroxymethylcytosine of 5-Hydroxymethylcytosine DNA.
IsotypeIgG1κ
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular WeightThis antibody detects 5-hydroxymethylcytosine.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Dot Blot in unmodified and modified cytosine DNA.

Dot Blot Analysis: 0.1 µg/mL of this antibody detected 5-hydroxymethylcytosine in 10 ng and 50 ng of unmodified and modified cytosine DNA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE251 04053252691188

Documentation

Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 SDS

Title

Safety Data Sheet (SDS) 

Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 Certificates of Analysis

TitleLot Number
Anti-5-hydroxymethylcytosine (5hmC), -2813511 2813511
Anti-5-hydroxymethylcytosine (5hmC), clone HMC 31 2919617
Anti-5-hydroxymethylcytosine (5hmC), clone HMC 31 - 2390570 2390570
Anti-5-hydroxymethylcytosine (5hmC), clone HMC 31 - 2429692 2429692
Anti-5-hydroxymethylcytosine (5hmC), clone HMC 31 - 2080897 2080897
Anti-5-hydroxymethylcytosine (5hmC), clone HMC 31 - 2246820 2246820
Anti-5-hydroxymethylcytosine (5hmC), clone HMC 31 - 3460375 3460375
Anti-5-hydroxymethylcytosine (5hmC), clone HMC 31 - 3819977 3819977
Anti-5-hydroxymethylcytosine (5hmC), clone HMC 31 - 3923736 3923736
Anti-5-hydroxymethylcytosine (5hmC), clone HMC 31 - Q2005669 Q2005669
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Brochure

Title
CpGenome™ 5-hmC Quantitation Kit
New Products: Volume 3, 2012