Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

132647 Alcian blue staining kit, pH 1.0

for the detection of strongly sulfated mucins for microscopy

Alcian blue staining kit, pH 1.0 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
132647
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number132647
DescriptionAlcian Blue Staining Kit, pH 1,0
OverviewThe ready-to-use Alcian blue stainig kit, pH 1.0 - for the detection of strongly sulfated mucins - for microscopy, can be used for this staining in histological tissue. Alcian blue pH 1.0 is used to stain strongly sulfated mucins in human-histological specimen materials, for example histological sections of e. g. colon or bronchus. The dye Alcian blue 8 GX (Cat. no.101647) is a water-soluble phthalocyanine dye. Acidic mucins have a negative charge and bind the positively charged Alcian blue 8 GX staining dye.
A solution of hydrochloric acid and Alcian blue is used for the staining procedure. At a strongly acidic pH of 1.0, this solution suppresses the dissociation of the carboxyl groups and selectively stains only the strongly sulfated mucins and polyanions. Counterstaining takes place with a nuclear fast red/aluminium sulfate solution. The undifferentiated method (Alcian blue 8 GX solution, pH 2.5) enables only the detection of acidic glycoproteins, e. g. mucins, without distinguishing between carboxyl and sulfate groups.
The package is sufficient for approx. 2500 applications. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.
References
Product Information
Quality LevelMQ400
Applications
Applicationfor the detection of strongly sulfated mucins for microscopy
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H290: May be corrosive to metals.
H318: Causes serious eye damage.
Precautionary Statement(s)P234: Keep only in original packaging.
P280: Wear eye protection/ face protection.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P390: Absorb spillage to prevent material damage.
Signal WordDanger
Storage class8A Combustible, corrosive hazardous materials
WGKWGK 2 obviously hazardous to water
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1789 , 8, III
Declaration (transport by air) IATA-DGRUN 1789 , 8, III
Declaration (transport by sea) IMDG-CodeUN 1789 , 8, III, Segregation Group: 1 (Acids)
Supplemental Information
Specifications