Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-453 Akt1/PKBα (δPH, S473D) Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant human Akt1, residues 118-end containing the mutation S473D, for use in Kinase Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
14-453
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-453M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-453
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAkt1/PKBα (δPH, S473D) Protein, active, 10 µg
OverviewN-terminal His6-tagged, recombinant human Akt1, residues 118-end containing the mutation S473D
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged, recombinant human Akt1, residues 118-end containing the mutation S473D, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman Akt1 expressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Gene Symbol
  • AKT1
  • RAC
  • PRKBA
  • MGC99656
  • RAC-ALPHA
  • RAC-PK-alpha
  • C-AKT
  • PKB
  • AKT
Protein TargetAkt1/PKBα
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight45kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of Crosstide substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-453M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-453 04053252627651

Documentation

Akt1/PKBα (δPH, S473D) Protein, active, 10 µg SDS

Title

Safety Data Sheet (SDS) 

Akt1/PKBα (δPH, S473D) Protein, active, 10 µg Certificates of Analysis

TitleLot Number
Akt1/PKB#945; (S473D), active - 1600485-H 1600485-H
Akt1/PKB#945; (S473D), activee - 1600485-G 1600485-G

FAQ

QuestionAnswer
What type of buffer is best for lysis when attempting to preserve a protein?The best buffer is Buffer A. It is composed of 50mM Tris/HCl, pH 7.5, 1mM EGTA, 1mM EDTA, 1mM Sodium Orthovanadate (activated), 10mM Na B-glycerolphosphate, 50mM NaF, 5mM Na pyrophosphate, 1% w/v Triton X-100, 0.1% w/v 2-mercaptoethanol, 1ug/ml each of aprotinin, leupeptin and pepstatin, 1mM PMSF and 1mM microcystin (this is optional). QS with water. You may store Buffer A in aliquots at -20°C for 6 months.
Some of my enzyme preparations require Brij-35, what is its purpose?The role of Brij-35 is to function as a mild detergent, which prevents aggregation in active kinases.

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-453M Akt1/PKBα (δPH, S473D) Protein, active, 250 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases