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114700 Activin A, Human, Recombinant, CHO Cells

114700
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Purity
≥95% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
114700-10UG Plastic ampoule 10 μg
Description
OverviewRecombinant, human activin A (disulfide-linked homodimer of two 116-amino acid residue βA subunits) expressed in CHO cells. Mature βA-monomer lacks a potential N-linked glycosylation site and has a calculated molecular weight of 13 kDa. A member of the TGF-β superfamily that is involved in the negative regulation of B lineage lymphocytes. Shown to play an important role in the regulation of hormonogenesis, cellular homeostasis, and differentiation programs. Reported to inhibit endogenous production of IL-6.
Catalogue Number114700
Brand Family Calbiochem®
References
ReferencesZipori, D., et al. 2001. J. Leukoc. Biol. 69, 867.
Hedger, M.P., et al. 2000. Cytokine 12, 595.
Woodruff, T.K. 1998. Biochem. Pharmacol. 55, 953.
Ying, S.Y., et al. 1997. Proc. Soc. Exp. Biol. Med. 214, 114.
Schwall, R.H., and Lei, C. 1991. Methods Enzymol. 198, 340.
Mason, A., et al. 1986. Biochem. Biophys. Res. Commun. 135, 957.
Product Information
FormLyophilized
FormulationLyophilized from sterile filtered 35% acetonitrile, 0.1% TFA, 50 µg BSA/µg Activin A.
Quality LevelMQ100
Biological Information
Biological activityED₅₀ = 0.5-2.0 ng/ml as measured by its ability to induce hemoglobin expression in K562 cells
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤100 pg/µg Activin A
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Global Trade Item Number
Catalogue Number GTIN
114700-10UG 04055977208085