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114666 Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem

Actinomycin D, Streptomyces sp., CAS 50-76-0, is an anti-neoplastic antibiotic that inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I

Primary Target: serine proteases
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
50-76-0C₆₂H₈₆N₁₂O₁₆
Description
OverviewAnti-neoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. At higher concentrations, DNA polymerase is inhibited. Also acts as a competitive inhibitor of serine proteases. Inhibits cell growth and colony formation in synchronized HeLa cells. Induces apoptosis in many cell lines. However, actinomycin D has also been shown to suppress etoposide-induced apoptosis in PC12 cells. Note: 1 set = 20 x 200 µg.
Stability: heat resistant and stable between pH 2 and 11. Decomposed by strong acids and bases.
Catalogue Number114666
Brand Family Calbiochem®
SynonymsDactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I
References
ReferencesNakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
Product Information
CAS number50-76-0
FormRed crystalline solid
Hill FormulaC₆₂H₈₆N₁₂O₁₆
Chemical formulaC₆₂H₈₆N₁₂O₁₆
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationActinomycin D, Streptomyces sp., CAS 50-76-0, is an anti-neoplastic antibiotic that inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues.
Biological Information
Primary Targetserine proteases
Secondary targetcell growth and colony formation in synchronized HeLa cells
Purity≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSAU1575000
Safety Information
R PhraseR: 28

Very toxic if swallowed.
S PhraseS: 28-36/37-45


Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic & Carcinogenic / Teratogenic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
114666 0

Documentation

Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem Certificates of Analysis

TitleLot Number
114666

References

Reference overview
Nakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Tools and Tips for Analyzing Apoptosis

Citations

Title
  • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
  • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
  • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision07-August-2009 JSW
    SynonymsDactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I
    DescriptionAntineoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. A DNA-intercalator with growth inhibitory activity against certain leukemias and sarcomas. At higher concentrations, DNA polymerase is inhibited. Inhibits cell growth and colony formation in synchronized HeLa cells. A potent inducer of apoptosis in many cell lines; however, actinomycin D has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II. Acts as a competitive inhibitor of serine protease from microorganisms. The growth of S. aureus is inhibited by 0.25-0.75 µg/ml, whereas a concentration of 100 µg/ml is required to inhibit E. coli due to permeability differences. Heat resistant and stable between pH 2 and 11; decomposed by strong acids and bases. λmax 441 nm ±2 nm. Note: 1 set = 20 vials x 200 µg.
    FormRed crystalline solid
    CAS number50-76-0
    RTECSAU1575000
    Chemical formulaC₆₂H₈₆N₁₂O₁₆
    Structure formulaStructure formula
    Purity≥98% by HPLC
    SolubilityDMSO (1 mg/ml), Methanol, or Chloroform
    Storage Protect from light
    +2°C to +8°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.
    Toxicity Highly Toxic & Carcinogenic / Teratogenic
    Merck USA index14, 2800
    ReferencesNakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
    Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
    Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
    Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
    Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
    Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
    White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
    Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
    Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
    Citation
  • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
  • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
  • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
    		•