Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
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Reference Paper: Multi-Parameter Dose Estimations in Radiation Biodosimetry Using the Automated Cytokinesis-Block Micronucleus Assay with Imaging Flow Cytometry. Cytometry A 2014; 10: 883-893.
The study of DNA repair mechanisms is important in the field of oncology where radiotherapy used to treat tumors induces double strand breaks (DSB). The DNA damage that occurs can be indirectly visualized with a microscope by immunostaining the repair proteins that are recruited to DSB foci. Amnis® imaging flow cytometry automatically and objectively collect thousands of images, quantifies the foci in a population of cells and determines dose response kinetics faster and easier than manual microscopy.
Quantifying Micronuclei in Irradiated Human Lymphocytes Using the ImageStream®X
The cytokinesis-block micronucleus (CBMN) assay is a well-established technique in biological dosimetry for estimating radiation doses by correlating the frequency of MN in binucleated cells (BNCs) to a calibrated dose in peripheral blood lymphocytes. Advanced masking techniques allow for automated imaging, identification and enumeration of fluorescently stained BNCs and MN in irradiated lymphocytes. Using features such as aspect ratio, shape ratio and fluorescence spot counting, rapid analysis can be performed on large numbers of cell images captured by the ImageStream®X. Data was generously provided by M. A. Rodrigues.
Quantitation of γ-H2AX foci in Irradiated Cells Using the ImageStream®X
Phosphorylated H2AX (γ-H2AX) facilitates recognition and repair of DNA double strand breaks (DSBs) that may occur from exposure to ionizing radiation. Staining irradiated cells for γ-H2AX reveals nuclear foci that are readily observed microscopically in a dose response manner. Irradiated cells were analyzed for the number of spots in the nuclear region using advanced masking techniques that identify the punctate staining. Morphological measurements employed in this analysis including object shape, size, and punctate fluorescence spot counting emphasizing the advantages of quantitative multiparametric image analysis on large numbers of cells provided with the ImageStream®X.
The study of DNA repair mechanisms is important in the field of oncology where radiotherapy used to treat tumors induces double strand breaks (DSB). The DNA damage that occurs can be indirectly visualized with a microscope by immunostaining the repair proteins that are recruited to DSB foci. Amnis® imaging flow cytometry automatically and objectively collect thousands of images, quantifies the foci in a population of cells and determines dose response kinetics faster and easier than manual microscopy.
