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SCR066 Quantitative Alkaline Phosphatase ES Characterization Kit

This Quantitative Alkaline Phosphatase Embryonic Stem (ES) Characterization Kit easily quantifies the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenien

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Key Applications: Stem Cell Culture
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SCR066
100 assays  
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      Overview

      Key Spec Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCR066
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionQuantitative Alkaline Phosphatase ES Characterization Kit
      OverviewMillipore’s Quantitative Alkaline Phosphatase ES Characterization Kit allows researchers to easily quantify the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay. This kit can be used in conjunction with Millipore’s Alkaline Phosphatase Detection kit (Cat. No. SCR004) to fully characterize the levels of alkaline phosphatase present within an embryonic stem cell culture.
      Background InformationEmbryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and are capable of unlimited, undifferentiated proliferation in vitro under appropriate cell culture conditions. Undifferentiated embryonic stem cells are characterized by high expression levels of stage-specific embryonic antigens (SSEA-1, 3 and 4), tumor rejection antigens (TRA-1-60 and TRA-1-81), OCT-4 and alkaline phosphatase. These undifferentiated markers are downregulated upon spontaneous or induced differentiation.

      Alkaline phosphatase (ALP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. The enzyme is present within all tissues of the body but is elevated in cells of the liver, kidney, bone, placenta, embryo and under specific disease states. Under alkaline conditions (pH>10), ALP can catalyze the hydrolysis of p-nitrophenylphosphate (p-NPP) into phosphate and p-nitrophenol, a yellow colored by-product of the catalytic reaction. The amount of p-nitrophenol produced is proportional to the amount of alkaline phosphatase present within the reaction. The amount of ALP can thus be reliably quantified by reading the amount of p-nitrophenol amassed after the catalytic reaction at 405 nm on a spectrophotometer.
      Product Information
      Components
      • p-NPP Substrate Concentrate (50X): (Part No. ES008-200UL) 200 μL
      • p-NPP Buffer: (Part No. ES011-10ML) 10 mL
      • Reaction Stop Solution: (Part No.CS200653) 5 mL
      • 1X Wash Solution: (Part No. CS200652) 2 X 125 mL
      • Recombinant Alkaline Phosphatase Standard: (Part No. CS200654) 0.5 μg (10 μg/mL)
      HS Code3822 19 90
      Quality LevelMQ100
      Applications
      ApplicationThis Quantitative Alkaline Phosphatase Embryonic Stem (ES) Characterization Kit easily quantifies the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Species Reactivity
      • Mammals
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Cardiac Stem Cells
      • Induced Pluripotent Stem Cells
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAll reagents are to be stored at 2∘ to 8∘C until expiration date. p-NPP substrate concentrate and p-NPP buffer are light sensitive and should not be exposed to light. Reaction stop solution contains 1N sodium hydroxide and is corrosive. Avoid skin contact and inhalation.
      Packaging Information
      Material Size100 assays
      Global Trade Item Number
      Catalogue Number GTIN
      SCR066 04053252660665