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342001 Procathepsin K, Human, Recombinant, E. coli

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342001
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342001-10UG
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      Description
      OverviewRecombinant, human procathepsin K (amino acids 19-329) (GenBank target symbol = S79895, ACC No. P43235) expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site. Cathepsin K, a member of the papain superfamily of cysteine proteinases, plays an important role in osteoclast-mediated bone resorption and collagen degradation. Cathepsin K is synthesized as an inactive proenzyme that is converted to its mature, active form by proteolytic cleavage of the 99 amino acid propeptide domain. Inhibitors of cathepsin K include leupeptin (Cat. No. 108975) (IC50 = 70 nM), E-64 (Cat. No. 324890) (IC50 = 5 nM), and cystatin (Cat. No. 324891 or 324896). Requires activation prior to use.
      If the activated enzyme is not used immediately, it is recommended to add methyl methanthiosulfonate (1 mM final concentration MMTS).
      Note: 1 mU = 1 milliunit.
      Catalogue Number342001
      Brand Family Calbiochem®
      References
      ReferencesMcQueney, M., et al. 1997. J. Bio. Chem. 272, 13955.
      Bossard, M., et al. 1996. J. Biol. Chem. 271, 12517.
      Drake, F., et al. 1996. J. Biol. Chem. 271, 12511.
      Bromme, D. and Okamoto, K. 1995. Biol. Chem. Hoppe-Seyler 376, 379.
      Baron, R. 1989 Anat. Rec. 224, 317.
      Littlewood-Evans, A.J., et al. 1975. Cancer Res. 57, 5386.
      Nishimura, J.S. et al. 1975. Arch. Biochem. Biophys. 170, 461.
      Product Information
      Activity≥1000 mU/mg protein
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmole benzyloxycarbonyl-phenylalanine-arginine-7-amido-4-methylcoumarin per min at 37°C, pH 5.5.
      EC number3.4.22.38
      FormLiquid
      FormulationIn 500 mM NaCl, 25 mM Tris, pH 8.0.
      Quality LevelMQ100
      Biological Information
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Following activation the enzyme is unstable and should include MMTS for storage (see recommended reaction conditions for activation).
      Global Trade Item Number
      Catalogue Number GTIN
      342001-10UG 04055977194999