342001 Sigma-AldrichProcathepsin K, Human, Recombinant, E. coli
Recombinant, human procathepsin K expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site.
More>> Recombinant, human procathepsin K expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site. Less<<Recommended Products
-
17-10460 Sigma-Aldrich Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit -
MABX4161-10KC Sigma-Aldrich Anti-Oxycodone (10mg) KC -
GM520FBE-SP326 Millipore SPARE PART KIT FBE 20MM,EP,DC -
203290 Sigma-Aldrich Bisindolylmaleimide I - CAS 133052-90-1 - Calbiochem -
8146920100 Sigma-Aldrich Hexadecyltrimethylammonium-p-toluenesulfonate -
220588-250MG Sigma-Aldrich Chlorophenolred-β-D-galactopyranoside, Sodium Salt -
KR99A10HH1 Millipore Opticap® XL10 Polygard®-CR 100 µm 9/16 in. HB/HB -
1191660001 Millipore Perforated lid alu 400x0.7 mm
Overview
Pricing & Availability
Catalogue Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
---|---|---|---|---|---|---|
342001-10UG |
|
Plastic ampoule | 10 μg |
|
— |
Description | |
---|---|
Overview | Recombinant, human procathepsin K (amino acids 19-329) (GenBank target symbol = S79895, ACC No. P43235) expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site. Cathepsin K, a member of the papain superfamily of cysteine proteinases, plays an important role in osteoclast-mediated bone resorption and collagen degradation. Cathepsin K is synthesized as an inactive proenzyme that is converted to its mature, active form by proteolytic cleavage of the 99 amino acid propeptide domain. Inhibitors of cathepsin K include leupeptin (Cat. No. 108975) (IC50 = 70 nM), E-64 (Cat. No. 324890) (IC50 = 5 nM), and cystatin (Cat. No. 324891 or 324896). Requires activation prior to use. |
If the activated enzyme is not used immediately, it is recommended to add methyl methanthiosulfonate (1 mM final concentration MMTS). | |
Note: 1 mU = 1 milliunit. | |
Catalogue Number | 342001 |
Brand Family | Calbiochem® |
Product Information | |
---|---|
Activity | ≥1000 mU/mg protein |
Unit of Definition | One unit is defined as the amount of enzyme that will hydrolyze 1 µmole benzyloxycarbonyl-phenylalanine-arginine-7-amido-4-methylcoumarin per min at 37°C, pH 5.5. |
EC number | 3.4.22.38 |
Form | Liquid |
Formulation | In 500 mM NaCl, 25 mM Tris, pH 8.0. |
Quality Level | MQ100 |
Biological Information | |
---|---|
Purity | ≥95% by SDS-PAGE |
Concentration Label | Please refer to vial label for lot-specific concentration |
Global Trade Item Number | |
---|---|
Catalogue Number | GTIN |
342001-10UG | 04055977194999 |