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559309 PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb

Overview

Key Spec Table

Species ReactivityHostAntibody Type
Nem, Ch, Dr, H, M, RRbPolyclonal Antibody

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559309-10T
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      Description
      OverviewRecognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr183 and Tyr185.
    • Antibody Target Gene Symbol: MAPK8, MAPK9
    • Target Synonym: AI849689, C-JUN N-TERMINAL KINASE1, JNK, JNK1, JNK1 PROTEIN KINASE, JNK1A2, JNK21B1/2, p46JNK1, p46JNK1 ALPHA, PRKM8, Sapk gamma, SAPK P46, SAPK1, SAPK1/JNK, STRESS-ACTIVATED PROTEIN KINASE-LIKE KINASE, AI851083, Jnk p54, Jnk p55, JNK-55, JNK2, JNK2A, Jnk2a2, JNK2ALPHA, JNK2B, JNK2BETA, JUNKINASE, p54a, p54aSAPK, PRKM9, SAPK, SAPK ALPHA, SAPK ALPHA 1
    • Entrez Gene Name: mitogen-activated protein kinase 8
    • Hu Entrez ID: 5601
    • Mu Entrez ID: 26420
    • Rat Entrez ID: 50658
    • Catalogue Number559309
      Brand Family Calbiochem®
      Application Data
      Detection of human JNK1 and JNK2, phosphorylated on Tyr183 and Thr185, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV irradiation (lanes 2-6). Primary antibody: PhosphoDetect™ Anti-SAPK/JNK (pTyr183,pThr185) Rabbit pAb (Cat. No. 559309) (1:1000) pre-incubated without peptide (lanes 1,6) or with immunogen phosphopeptide (lane 2), a generic phosphothreonine-containing peptide (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or non-phosphopeptide corresponding to the immunogen (lane 5). Detection: chemiluminescence.
      References
      ReferencesFanger, G.R., et al. 1999. Histol. Histopathol. 14, 887.
      Gabai, V.L., et al. 1998. FEBS Lett. 438, 1.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesImmunoblotting (1:1000)
      Immunohistochemistry (see comments)
      Application CommentsSpecific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Nematode
      • Chicken
      • Drosophila
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      559309-10T 04055977192445