Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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561306
Sigma-AldrichP-Selectin, Human, Recombinant, CHO Cells
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Description
Overview
Recombinant, human P-selectin consisting of 730 amino acids minus the transmembrane and cytoplasmic domains expressed in CHO cells. This glycoprotein is expressed on activated platelets and endothelial cells. A specific interaction between P-selectin and P-selectin glycoprotein ligand-1, present on numerous cell types, promotes adhesion and acts along with L-selectin in establishing an initial interaction between circulating leukocytes and the endothelium.
Catalogue Number
561306
Brand Family
Calbiochem®
Synonyms
CD62P, GMP-140, LECAM-3, PADGEM
References
Product Information
Form
Lyophilized
Formulation
Lyophilized from Dulbecco’s D-PBS (containing calcium and magnesium), 1% sorbitol.
P-Selectin, Human, Recombinant, CHO Cells Certificates of Analysis
Title
Lot Number
561306
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-September-2008 RFH
Synonyms
CD62P, GMP-140, LECAM-3, PADGEM
Description
Recombinant, human P-selectin consisting of 730 amino acids minus the transmembrane and cytoplasmic domains expressed in CHO cells. This glycoprotein is expressed on activated platelets and endothelial cells. A specific interaction between P-selectin and P-selectin glycoprotein ligand-1, present on numerous cell types, promotes adhesion and acts along with L-selectin in establishing an initial interaction between circulating leukocytes and the endothelium.
Form
Lyophilized
Formulation
Lyophilized from Dulbecco’s D-PBS (containing calcium and magnesium), 1% sorbitol.
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxins: <1.5 EU/µg of P-Selectin
Biological activity
Shown to bind to P-selectin ligands on U937 cells.
Solubility
Reconstitute at 1 mg/ml in sterile H₂O.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
