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Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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411550
Sigma-AldrichMouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate
This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.
More>>This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Enzyme Immunoassay
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Cross-Reactivity, Myeloma Proteins
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Key Applications
Enzyme Immunoassay
Application Notes
Enzyme Immunoassay (1:1000, see application references)
Application Comments
Antibody should be titrated for optimal results in individual systems.
Cross-reactivity by ELISA against human myeloma proteins: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: <0.2% Human IgA: <0.1% Human IgE: <0.1%
Cross-reactivity by ELISA against chimeric antibodies: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: <0.01% Human IgA: <0.01% Human IgE: <0.01%
There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Biological Information
Clone
HP6043
Host
Mouse
Isotype
IgG
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Global Trade Item Number
Catalogue Number
GTIN
411550-500UG
07790788060275
Documentation
Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate SDS
Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate Certificates of Analysis
Title
Lot Number
411550
References
Reference overview
Hamilton, R.G. 1993. Clin. Chem.39, 1988. Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107. Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473. Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275. Hamilton, R.G., et al. 1987. Hybridoma6, 204. Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
11-March-2009 JSW
Application
Enzyme Immunoassay (1:1000, see application references)
Description
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
Background
The HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the CALBIOCHEM® technical monograph, The Human IgG Subclasses, is available free of charge.
Host
Mouse
Clone
HP6043
Isotype
IgG
Form
Liquid
Formulation
In PBS, 10 mg/ml BSA.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
None
Comments
Antibody should be titrated for optimal results in individual systems.
Cross-reactivity by ELISA against human myeloma proteins: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: <0.2% Human IgA: <0.1% Human IgE: <0.1%
Cross-reactivity by ELISA against chimeric antibodies: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: <0.01% Human IgA: <0.01% Human IgE: <0.01%
There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Toxicity
Standard Handling
References
Hamilton, R.G. 1993. Clin. Chem.39, 1988. Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107. Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473. Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275. Hamilton, R.G., et al. 1987. Hybridoma6, 204. Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.
Application references
Enzyme Immunoassay
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Cross-Reactivity, Myeloma Proteins
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
