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FCMAB313PE Milli-Mark® Anti-Nestin-PE Antibody, clone 10C2

Detect Nestin using this Milli-Mark Anti-Nestin-PE Antibody, clone 10C2 validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Neural Stem Cells Gene Symbol: NES, Nbla00170 UniProt Number: P48681
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FCMAB313PE
100 tests  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMPhycoerythrinMonoclonal Antibody
      Description
      Catalogue NumberFCMAB313PE
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark® Anti-Nestin-PE Antibody, clone 10C2
      Background InformationNestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
      Product Information
      FormatPhycoerythrin
      Control
      • U251 cells
      PresentationPurified mouse monoclonal IgG1κ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationDetect Nestin using this Milli-Mark Anti-Nestin-PE Antibody, clone 10C2 validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenFusion protein
      EpitopeHuman
      Clone10C2
      HostMouse
      SpecificityAntibody recognizes Human Nestin.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteTested and passed on human. No reactivity with mouse or rat Nestin.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
      Gene Symbol
      • NES
      • Nbla00170
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).

      SIZE: 1621 amino acids; 177439 Da

      SUBUNIT: Interacts with FHOD3 (By similarity).

      TISSUE SPECIFICITY: CNS stem cells.

      DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

      SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.
      Molecular Weight177 kDa Calculated
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using U251 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Global Trade Item Number
      Catalogue Number GTIN
      FCMAB313PE 04053252294624