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109261 Lugol's solution (diluted iodine-potassium iodide solution)

for the Gram staining method

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Iodine-potassium iodide solution

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109261
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1092611000
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      		 Glass bottle 1 l
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      1092612500
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          		 Glass bottle 2.5 l
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          Description
          Catalogue Number109261
          SynonymsIodine-potassium iodide solution
          DescriptionLugol's solution (diluted iodine-potassium iodide solution)
          OverviewLugol’s solution (diluted iodine-potassium iodide solution - for the Gram staining method, is a ready-to-use solution used in Bacteriology, for example, for the staining of Gram bacteria. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet (Cat.no. 109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution), a dye-iodine complex will form. During the decolorizing step (Gram's decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Cat.no. 109217) and will then appear pink to red.
          Lugol's solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          HS Code3212 90 90
          Quality LevelMQ400
          Applications
          Applicationfor the Gram staining method
          Biological Information
          Physicochemical Information
          Boiling point100 °C (1013 hPa)
          Density1.01 g/cm3 (20 °C)
          pH value3.5 (H₂O, 20 °C)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Storage class10 - 13 Other liquids and solids
          WGKWGK 2 obviously hazardous to water
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Suitability for microscopypasses test
          gram-positve microorganismsblue-violet
          gram-negative microorganismspink to red
          Global Trade Item Number
          Catalogue Number GTIN
          1092611000 04022536112701
          1092612500 04022536557410

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          Categories

          IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining