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CC1019 Human Integrin αVβ3, Triton X-100 Formulation

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Positive Control UniProt Number: P06756 Entrez Gene Number: NM_002210.2
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CC1019
50 µg  
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      Overview

      Description
      Catalogue NumberCC1019
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Integrin αVβ3, Triton X-100 Formulation
      OverviewIntegrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to aVb3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described (Belkin, 1990).
      Alternate Names
      • CD51/CD61
      Product Information
      PresentationPurified protein in 20 mM Tris-HCI, pH 7.5, 150 mM NaCI, 2 mM MgCI2, 0.2% Triton X-100. No preservatives.
      Quality LevelMQ100
      Applications
      Key Applications
      • Positive Control
      Application NotesElectrophoresis and Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Concentration0.2 mg/mL
      Purity> 95% pure by gel analysis. Nonreduced 7.5% PAAG stained with Silver stain shows major bands at 92 kDa (beta3 subunit), 145 kDa (alphaV subunit) and a minor band at 170 kDa (beta3 subunit dimer).
      Specific ActivityFunctional activity confirmed by interaction of integrin alphaV/beta3 with vitronectin, von Willebrand Factor, fibrinogen, and fibronectin in RGD-dependent ELISA testing.
      Entrez Gene Number
      Entrez Gene SummaryITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
      Gene Symbol
      • ITGAV
      • DKFZp686A08142
      • MSK8
      • CD51
      • VNRA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1048 amino acids; 116038 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -70°C in undiluted aliquots. Avoid repeated freeze/ thaw cycles.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Catalogue Number GTIN
      CC1019 04053252620584