Millipore Sigma Vibrant Logo

PMEF-CFX EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Irradiated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
View Products on Sigmaaldrich.com
PMEF-CFX
5 vials  5-6x106 ea
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Fulfillment and delivery delayed Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      Interested in purchasing an entire lot of MEF’s?
      Bulk sized lots of MEFs are available with lot sampling.
      Order Today

      Key Spec Table

      Key Applications
      Cult
      Description
      Catalogue NumberPMEF-CFX
      Trade Name
      • Embryomax
      • Specialty Media
      DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
      OverviewThese PMEF cells were isolated from CF-1 mouse embryos at day 13 and mitotically inactivated by treatment with irradiation. The cells can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:

      • 6 x 60mm dishes
      • 2 x T75 flasks
      • 2 x 6 well plates

      Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells.
      Alternate Names
      • MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells, Irradiated MEFS
      Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
      References
      Product Information
      HS Code3002 15 90
      Quality LevelMQ100
      Applications
      ApplicationPMEF, Strain CF1, Irradiated, Passage 3
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • PMEFs
      • Induced Pluripotent Stem Cells
      Cell Line Type
      • Primary Mouse Embryo Fibroblasts
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsVials should be stored in the vapor phase of liquid nitrogen.
      Packaging Information
      Material Size5 vials
      Material Package5-6x106 ea
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PMEF-CFX 04053252923661

      Documentation

      EmbryoMax® Primary Mouse Embryonic Fibroblasts SDS

      Title

      Safety Data Sheet (SDS) 

      EmbryoMax® Primary Mouse Embryonic Fibroblasts Certificates of Analysis

      TitleLot Number
      EmbryoMax#174; PMEF P3 Strain CF-1, Irradiated - RD1303001-1 RD1303001-1
      EmbryoMax#174; PMEF P3 Strain CF-1, Irradiated - RD1304013-1 RD1304013-1
      EmbryoMax® PMEF P3 Strain CF-1, Irradiated Primary Mouse Embryo Fibroblasts - 2440297-1 2440297-1
      EmbryoMax® PMEF P3 -2495246-1 2495246-1
      EmbryoMax® PMEF P3 -2513168-1 2513168-1
      EmbryoMax® PMEF P3 -2513168-3 2513168-3
      EmbryoMax® PMEF P3 -2706658-1 2706658-1
      EmbryoMax® PMEF P3 -2735010-1 2735010-1
      EmbryoMax® PMEF P3 Strain CF-1, Irradiated - 2463121-1 2463121-1
      EmbryoMax® PMEF P3 Strain CF-1, Irradiated - 2990407-2 2990407-2
      Show more

      Technical Info

      Title
      The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling