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EPA100 EPA Peroxidase Protection Buffer

500 mL EPA Peroxidase Protection Buffer for ELISA.

EPA Peroxidase Protection Buffer MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA
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EPA100
500 mL  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      ELISA
      Description
      Catalogue NumberEPA100
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionEPA Peroxidase Protection Buffer
      OverviewPeroxidase Protective Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods.
      References
      Product Information
      PresentationPeroxidase Protective Buffer contains 0.5% (w/v) bovine serum albumin, antimicrobial preservatives and stabilizing compounds in phosphate buffered saline, pH 7.2.
      Applications
      Application500 mL EPA Peroxidase Protection Buffer for ELISA.
      Key Applications
      • ELISA
      Application NotesUSE:

      Chemicon HRP conjugated antibodies may be diluted up to 1:16,000 in Peroxidase Protective Buffer. Diluted conjugates are stable for at least 3 months when stored at 2 - 8oC. The buffer is suitable for direct use in ELISA. It is recommended that the HRP conjugated antibody is diluted with Peroxidase Protective Buffer and allowed to stand for 24 hours at 2-8°C to attain constant stability before use.

      Peroxidase Protective Buffer is compatible with blocking agents such as skim milk powder, Tween 20 and normal serum. However HRP conjugates should not be stored in the presence of these blocking agents. They should be added just prior to use in the assay.
      Biological Information
      Physicochemical Information
      Dimensions
      Volume500 mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at +2 to +8°C, for up to 6 months.

      PRECAUTIONS

      HRP conjugates should be diluted and stored under clean conditions to reduce the level of microbial challenge.

      Sodium azide must NOT be added to Peroxidase Protective Buffer.

      Avoid contamination of HRP conjugate solutions with heavy metal ions, aromatic chlorocarbons or hypochlorous acids. Avoid containers made of soda glass or polystyrene. Storage of diluted conjugates in borosilicate glass is recommended.

      Efficient washing steps should follow HRP conjugate incubations. In ELISA, at least four thorough wash cycles are recommended.
      Packaging Information
      Material Size500 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      EPA100 04053252678509

      Documentation

      EPA Peroxidase Protection Buffer SDS

      Title

      Safety Data Sheet (SDS) 

      EPA Peroxidase Protection Buffer Certificates of Analysis

      TitleLot Number
      EPA100 - 08051489354 08051489354
      PEROXIDASE PROTECTIVE BUFFER -
      PEROXIDASE PROTECTIVE BUFFER - 3274769 3274769
      PEROXIDASE PROTECTIVE BUFFER - 3466088 3466088
      PEROXIDASE PROTECTIVE BUFFER - 3607348 3607348
      PEROXIDASE PROTECTIVE BUFFER - 3814362 3814362
      PEROXIDASE PROTECTIVE BUFFER - 3853990 3853990
      PEROXIDASE PROTECTIVE BUFFER - 4135719 4135719
      PEROXIDASE PROTECTIVE BUFFER -2561040 2561040
      PEROXIDASE PROTECTIVE BUFFER -2631125 2631125
      Show more

      Data Sheet

      Title
      PEROXIDASE PROTECTIVE BUFFER CERTIFICATE OF ANALYSIS