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CC162-350UG ECMatrix™-411 E8 Laminin Substrate

Xeno-free laminin-411 coating for the differentiation of pluripotent stem cells into endothelial cells, 350 µg (CHO-S derived)

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CC162-350UG
350 µg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberCC162-350UG
      DescriptionECMatrix™-411 E8 Laminin Substrate
      OverviewEndothelial cells line the interior surface of blood and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of tissue or organ. Laminin-411 is known to bind to the integrin α6β1 which is located on the cell surface of pluripotent stem cells and is known to induce endothelial cell differentiation. Professor Kiyotoshi Sekiguchi’s group (Matrixome, Inc.) have produced a recombinant E8 fragment of laminin-411 at large-scale while retaining the full integrin binding activity. ECMatrix™-411 E8 Laminin Substrates are recombinant Laminin-411-E8 fragments which bind to integrin α6β1 and increases the differnetiuation of pluripotent stem cells (ES/iPSCs) into acetyl-LDL+/ CD31+ endothelial cells.

      References
      1. Takayama K, et al. Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochem Biophys Res Commun. 2016 May 20;474(1):91-96.
      2. Ohta R, et al. Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Sci Rep. 2016 Nov 2;6:35680.
      Alternate Names
      • iMatrix-411
      Background InformationProtocol

      Precoating Method
      1. Dilute the 0.5 mg/mL stock solution with sterile PBS to achieve a 2.5 ug/mL working solution.
      2. Coat dishes with ECMatrix™-411 at 0.25 ug/cm2 (for example, for one well of a 6-well plate add 1 mL of the 2.5 ug/mL working solution).
      3. Incubate for 1 hour at 37°C, 3 hours at room temperate or overnight at 4°C.
      4. Before use, remove remaining fluid from the coated surface (do not rinse).
      5. Detach cells into small clumps using Accutase.
      6. Plate the cells at desired density.

      Note: Do not allow the plates to dry, briefly spin down all liquids in the tube before use, avoid repeated freeze-thaw cycles.
      References
      Product Information
      Components
      • 1) 2 X 175 µg ECMatrix™-411 E8 Laminin Substrate (0.5 mg/mL in PBS). Expressed in CHO-S cells.
      Applications
      ApplicationXeno-free laminin-411 coating for the differentiation of pluripotent stem cells into endothelial cells, 350 µg (CHO-S derived)
      Key Applications
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Purity (SDS-Page): > 95%
      • Endotoxin Test: ≤ 750 EU/mg
      • Mycoplasma Test: Negative
      • Sterility Test: Negative
      • Integrin Binding Assay (kDa): ≤ 10 nM
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsECMatrix™-411 E8 Laminin Substrates should be stored at 2-8°C. Avoid multiple freeze-thaw cycles and protect from light.
      Packaging Information
      Material Size350 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CC162-350UG 04061841030249