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MABE263-AF647 Anti-unmethylated Histone H3 (Lys9) Antibody, clone 9B1-2G6, Alexa Fluor® 647 Conjugate

This Anti-unmethylated Histone H3 (Lys9) Antibody, clone 9B1-2G6 is validated for use in Immunocytochemistry for the detection of unmethylated Histone H3 (Lys9).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Histones, Chromatin Biology Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ UniProt Number: P68431
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MABE263-AF647
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue NumberMABE263-AF647
      DescriptionAnti-unmethylated Histone H3 (Lys9) Antibody, clone 9B1-2G6, Alexa Fluor® 647 Conjugate
      Alternate Names
      • Histone H3.1
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/I
      • Histone
      • H3/j
      • Histone H3/k
      • Histone H3/l
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2. While lysine demethylation is mediated by enzymes such as JmjC domain-containing proteins.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified conjugated mouse monoclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
      Quality LevelMQ300
      Applications
      ApplicationThis Anti-unmethylated Histone H3 (Lys9) Antibody, clone 9B1-2G6 is validated for use in Immunocytochemistry for the detection of unmethylated Histone H3 (Lys9).
      Key Applications
      • Immunocytochemistry
      Application NotesSee data sheet for Cat. No. MABE263, Anti-unmethylated Histone H3 (Lys9), clone 9B1-2G6, for additional Western blot and dot blot specificity analysis performed using an unconjugated version of this antibody.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human unmethylated Histone H3 (Lys9).
      Clone9B1-2G6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThis antibody recognizes Histone H3 unmethylated at Lys9. This antibody does not cross react with methyl-Histone (Lys29). This antibody detects unmethylated Histone H3 (Lys9) when Ser10 is phosphorylated.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~15 kDa observed. Molecular weight was observed in parent product MABE263
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunocytochemistry Analysis:

      A 1:50 dilution of this antibody detected unmethylated Histone H3 (Lys9) in HeLa cells.
      Alexa Fluor® is a registered trademark of Life Technologies®.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE263-AF647 04053252983733