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04-816-I-100UL Anti-phospho-PPARgamma (Ser112) Antibody, clone AW504 rabbit monoclonal

Anti-phospho-PPARgamma (Ser112), clone AW504 rabbit monoclonal, Cat. No. 04-816-I, is a highly specific rabbit monoclonal antibody that targets Peroxisome proliferator-activated receptor gamma and has been tested for use in Western Blotting.

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04-816-I-100UL
100 μL  
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      Overview

      Replacement Information
      Description
      Catalogue Number04-816-I-100UL
      DescriptionAnti-phospho-PPARgamma (Ser112) Antibody, clone AW504 rabbit monoclonal
      Alternate Names
      • Peroxisome proliferator-activated receptor gamma
      • Nuclear receptor subfamily 1 group C member 3
      Background InformationPeroxisome proliferator-activated receptor gamma (UniProt: P37231; also known as PPAR-gamma, Nuclear receptor subfamily 1 group C member 3) is encoded by the PPARG (also known as NR1C3) gene (Gene ID: 5468) in human. Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor family of transcription factors that mediate a variety of cellular processes, including glucose and lipid metabolism, inflammatory responses, and regulation of apoptotic cell death. They act by binding to specific peroxisome proliferator-response elements (PPREs) on target genes. Three types of PPARs exist, namely alpha, delta, and gamma. In addition, alternative splicing produces three PPAR-gamma isoforms. All PPARs heterodimerize with retinoid X receptor (RXR) and bind PPREs in the promoter region of target genes. Highest expression of PPAR gamma is found in the adipose tissue and lower levels are detected in skeletal muscle, spleen, heart and liver. It plays a critical role in adipocyte differentiation and fat deposition. PPAR-gamma is phosphorylated in basal conditions and is dephosphorylated upon treatment with ligands. Its dephosphorylation at serine 112 induces its adipogenic activity. PPAR-gamma can be activated by prostaglandin PGJ2 and certain members of the 5-HETE family of arachidonic acid metabolites, including 5-oxo-15(S)-HETE and 5-oxo-ETE.
      References
      Product Information
      FormatCulture Supernatant
      PresentationRabbit monoclonal antibody in cell culture supernatant with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-PPARgamma (Ser112), clone AW504 rabbit monoclonal, Cat. No. 04-816-I, is a highly specific rabbit monoclonal antibody that targets Peroxisome proliferator-activated receptor gamma and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids surrounding phosphoserine 112 from human PPAR gamma, isoform 2.
      CloneAW504
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityClone AW504 is a rabbit monoclonal antibody that specifically detects PPAR gamma phosphorylated on serine 112.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Canine, Human, Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PPARG
      • NR1C3
      Modifications
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~55 and 58 kDa observed; 57.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3/L1 cells differentiated into adipocyte-like cells.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-PPARgamma (Ser112) in NIH3T3/L1 cells differentiated into adipocyte-like cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      04-816-I-100UL 04054839961670

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