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06-500 Anti-p130 Cas Antibody

06-500
250 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-500
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-p130 Cas Antibody
      Overviewhas been referred to as anti-Cas3
      Product Information
      FormatPurified
      Presentation0.1M Tris-Glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide
      Applications
      ApplicationDetect p130 Cas using this Anti-p130 Cas Antibody validated for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST-fusion protein containing amino acids 691-799 of rat p130 Cas
      HostRabbit
      Specificityp130 Cas
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BCAR1
      • CAS
      • p130CAS
      • Crkas
      • P130Cas
      • CRKAS
      • p130cas
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P56945 # Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
      SIZE: 870 amino acids; 93362 Da
      SUBUNIT: Forms complexes in vivo with focal adhesion kinase 1, adapter protein CRKL and LYN kinase. Can heterodimerize with CASL. Interacts with BCAR3, NPHP1, PTK2B and SH2D3C (By similarity). Interacts with activated CSPG4. Interacts with INPPL1/SHIP2.
      SUBCELLULAR LOCATION: Cell junction, focal adhesion (By similarity). Cytoplasm (By similarity). Note=Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation (By similarity).
      TISSUE SPECIFICITY: Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
      DOMAIN: "SwissProt: P56945 Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2- binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL (By similarity). & A serine-rich region promotes activation of the serum response element (SRE). & The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.
      PTM: Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix.
      SIMILARITY: Belongs to the CAS family. & Contains 1 SH3 domain.
      Molecular Weight130kDa
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates of human A431 carcinoma cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size250 µg
      Global Trade Item Number
      Catalogue Number GTIN
      06-500 04053252627279