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MABS2773-25UG Anti-c-Kit/CD117 Antibody, clone H2C7

Anti-c-Kit/CD117, clone H2C7, Cat. No. MABS2773, is an Armenian hamster monoclonal antibody that detects c-Kit/CD117 and is tested for use in Immunocytochemistry and Immunohistochemistry.

NewMABS2773-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2773-25UG
      DescriptionAnti-c-Kit/CD117 Antibody, clone H2C7
      Alternate Names
      • Mast/stem cell growth factor receptor Kit
      • EC:2.7.10.1
      • SCFR
      • Proto-oncogene c-Kit
      • Tyrosine-protein kinase Kit
      • CD117
      Background InformationMast/stem cell growth factor receptor Kit (UniProt: P05532; also known as EC:2.7.10.1, SCFR, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117) is encoded by the Kit (also known as SI) gene (Gene ID: 16590) in murine species. c-Kit is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-527), a transmembrane domain (aa 528-548), and a cytoplasmic domain (aa 549-979). c-Kit has tyrosine protein kinase activity and serves as a cell-surface receptor for the stem cell factor (SCF)/KITLG. It plays a significant role in the regulation of cell survival, cell proliferation, stem cell maintenance, gametogenesis, and other functions. In response to SCF binding, c-Kit can activate several signaling pathways. In the absence of bound SCF, it exists as a monomer, however, it homodimerizes in the presence of bound SCF and forms a heterotetramer with two SCF molecules. c-Kit can be autophosphorylated on tyrosine residues and SCF binding enhances its autophosphorylation. Its kinase activity is reported to be down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at tyrosine 571 is shown to be essential for its interaction with PTPN11/SHP-2 and phosphorylation of tyrosine 573 is required for interaction with PTPN6/SHP-1. c-Kit can be ubiquitinated by SOCS6 and it is rapidly ubiquitinated after autophosphorylation induced by SCF binding, leading to its internalization and degradation. Lack of c-Kit expression has been reported in multiple cancer types and c-Kit expressing cells display a regression of typical morphological features of malignancy. c-kit expression is also reported in peptidergic small-sized dorsal root ganglia neurons and is involved peripherally and centrally in pain regulation. (Ref.: Foster, BM., et al. (2018). Biomedicines. 6(1); 31; Takagi, K., et al. (2008). Neuroscience. 153(4); 1278-1288; Koma, Y., et al. (2005). Lab. Invest. 85; 426-435; Yee, NS., et al. (1994). J. Biol. Chem. 269(50); 1991-1998).
      References
      Product Information
      FormatPurified
      PresentationPurified armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-c-Kit/CD117, clone H2C7, Cat. No. MABS2773, is an Armenian hamster monoclonal antibody that detects c-Kit/CD117 and is tested for use in Immunocytochemistry and Immunohistochemistry.
      Key Applications
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesTested Applications

      Immunohistochemistry Analysis: A 1:25 dilution from a representative lot detected c-Kit/CD117 in developing mouse forebrain frozen tissue sections.

      Immunocytochemistry Analysis: A representative lot detected c-Kit/CD117 in Immunocytochemistry applications (Koma, Y., et al. (2005). Lab Invest. 85(3):426-35).

      Immunohistochemistry Applications: A representative lot detected c-Kit/CD117 in Immunohistochemistry applications (Yamatani, H., et al. (2004). J Comp Neurol. 475(2):247-60; Takagi, K., et al. (2008). Neuroscience. 153(4):1278-88).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenE14 mouse olfactory cortices.
      EpitopeExtracellular domain
      CloneH2C7
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostArmenian Hamster
      SpecificityClone H2C7 is an Armenian hamster monoclonal antibody that detects c-Kit/CD117. It targets an epitope within the extracellular domain.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Kit
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight109.34 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in E14 Mouse liver sections.

      Immunohistochemistry Applications: A 1:25 dilution from a representative lot detected c-kit/CD117 in E14 Mouse liver frozen tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2773-25UG 04065270937957